Recombinant Mouse IL-18/IL-1F4 Protein

Name: Recombinant Mouse IL-18/IL-1F4 Protein
Brand: R&D Systems
SKU: 9139-IL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9139-IL

Citations (8)

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Key Product Details

Source:	E. coli
Accession #:	P70380
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-18/IL-1F4 Protein

Product Specifications for Recombinant Mouse IL-18/IL-1F4 Protein

Source	E. coli-derived mouse IL-18/IL-1F4 protein Asn36-Ser192
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asn36 & Leu40
Predicted Molecular Mass	18 kDa
SDS-PAGE	17 kDa, reducing conditions
Activity	Measured by its ability to induce mouse IFN-gamma secretion by activated mouse T cells. The ED₅₀ for this effect is 0.06-0.36 ng/mL.

Scientific Data Examples for Recombinant Mouse IL-18/IL-1F4 Protein

	Recombinant Mouse IL-18/IL-1F4 Protein Bioactivity Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) induces IFN gamma secretion by activated mouse T cells. The ED₅₀ for this effect is 0.06-0.36 ng/mL.
	Recombinant Mouse IL-18/IL-1F4 Protein SDS-PAGE 1 μg/lane of Recombinant Mouse IL-18 (Catalog # 9139-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

9139-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9139-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8⁺ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4⁺ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).

Item	Value
References	Dinarello, C.A. et al. (2013) Front. Immunol. 4:289. Smith, D.E. (2011) J. Leukoc. Biol. 89:383. Gu, Y. et al. (1997) Science 275:206. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Cheung, H. et al. (2005) J. Immunol. 174:5351. Novick, D. et al. (1999) Immunity 10:127. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769. Lalor, S.J. et al. (2011) J. Immunol. 186:5738. Li, J. et al. (2004) Cell. Immunol. 227:103. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665. Okamura, H. et al. (1995) Nature 378:88.
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Recombinant Mouse IL-18/IL-1F4 Protein (8)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-18/IL-1F4 Protein include:

Species: Mouse
Sample Types: Organoids
Applications: Bioassay

HY Chiang et al. (2022), IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence Nature Communications, 13(1):874.
PMID: 35169117
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Zhao et al. (2022), Inï¿½vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-gamma secretion by natural killer cells Cell Reports, 40(11):111342.
PMID: 36103837
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

PT Rudak et al. (2021), Chronic stress physically spares but functionally impairs innate-like invariant Tï¿½cells Cell Reports, 35(2):108979.
PMID: 33852855
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

G Zhao et al. (2021), Interleukin-18 accelerates cardiac inflammation and dysfunction during ischemia/reperfusion injury by transcriptional activation of CXCL16 Cellular Signalling, 0(0):110141.
PMID: 34487815
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

JA Moral et al. (2020), ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity Nature, 0(0).
PMID: 32076273
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

C Ge et al. (2019), Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment Cell Rep, 29(13):4236-4244.e3.
PMID: 31875535
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

IJ Jensen et al. (2018), Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections PLoS Pathog., 14(10):e1007405.
PMID: 30379932
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Jonas J Staudacher et al. (2017), Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target. Scientific Reports, 0(0):2045-2322.
PMID: 28986573

Recombinant Mouse IL-18/IL-1F4 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse IL-18/IL-1F4 Protein

Scientific Data Examples for Recombinant Mouse IL-18/IL-1F4 Protein

Formulation, Preparation and Storage

Carrier Free

9139-IL

Carrier

9139-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-18/IL-1F4

Citations for Recombinant Mouse IL-18/IL-1F4 Protein (8)

Product Documents for Recombinant Mouse IL-18/IL-1F4 Protein

Product Datasheets

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