What is the difference between the two versions of Recombinant Human IL-18, Catalog # 9124-IL and Catalog # B001-5?

Name: Recombinant Human IL-18/IL-1F4 Protein
Brand: R&D Systems
SKU: B001-5

Catalog # 9124-IL is manufactured by R&D Systems, while Catalog # B001-5 is manufactured by MBL. Catalog # 9124-IL is lyophilized powder whereas Catalog # B001-5 is supplied in solution (PBS with BSA and sucrose). Activity of the two proteins is measured in similar cell-based assays; please consult the respective product-specific pages for assay details. Catalog # 9124-IL is competitively priced and has higher purity and lower endotoxin than Catalog # B001-5.

Recombinant Human IL-18/IL-1F4 Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # B001-5

Citations (39)

Product Datasheet / COA / SDS

New Version Available - 9124-IL

A New rh IL-18 is Now Available! Competitively priced; Higher purity; Lower endotoxin; Manufactured in-house!

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-18/IL-1F4 Protein

Product Specifications for Recombinant Human IL-18/IL-1F4 Protein

Source	E. coli-derived human IL-18/IL-1F4 protein 37-193 aa
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass	18 kDa
Activity	Induction of IFN-gamma by KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant human IL-18 was measured using human IFN-gamma ELISA. The activity of lot 111 was as follows: IL-18 final concentration 10 ng/mL; IFN-gamma induction 48.1 IU/mL and IL-18 final concentration 20 ng/mL; IFN-gamma induction 64.3 IU/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

B001-5

Formulation	25 μg in PBS containing 0.1% BSA and 1% sucrose.
Reconstitution	Reconstitute in 250 μL of ice-cold distilled water on ice. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8⁺ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4⁺ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

Item	Value
References	Dinarello, C.A. et al. (2013) Front. Immunol. 4:289. Smith, D.E. (2011) J. Leukoc. Biol. 89:383. Gu, Y. et al. (1997) Science 275:206. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Cheung, H. et al. (2005) J. Immunol. 174:5351. Novick, D. et al. (1999) Immunity 10:127. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769. Lalor, S.J. et al. (2011) J. Immunol. 186:5738. Li, J. et al. (2004) Cell. Immunol. 227:103. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665. Ushio, S. et al. (1996) J. Immunol. 156:4274. Gaggero, A. et al. (2004) Oncogene 23:7552. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Recombinant Human IL-18/IL-1F4 Protein (39)

Showing 1 - 10 of 39 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-18/IL-1F4 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LD Uppendahl et al. (2019), Cytokine-induced memory-like natural killer cells have enhanced function, proliferation, and in vivo expansion against ovarian cancer cells Gynecol. Oncol., 0(0).
PMID: 30658847
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TA Mace et al. (2019), Soy isoflavones and their metabolites modulate cytokine-induced natural killer cell function Sci Rep, 9(1):5068.
PMID: 30911044
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

RP Semper et al. (2019), Helicobacter pylori Exploits the NLRC4 Inflammasome to Dampen Host Defenses J. Immunol., 0(0).
PMID: 31511355
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Hofland et al. (2019), Dissection of the Effects of JAK and BTK Inhibitors on the Functionality of Healthy and Malignant Lymphocytes J. Immunol., 0(0).
PMID: 31511358
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CL Zimmer et al. (2019), NK cells are activated and primed for skin-homing during acute dengue virus infection in humans Nat Commun, 10(1):3897.
PMID: 31467285
Species: Human
Sample Types: Whole Blood
Applications: Bioassay

S Suliman et al. (2019), MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria J. Immunol., 0(0).
PMID: 31611259
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Han et al. (2018), Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry Nat Protoc, 0(0).
PMID: 30258176
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Golden-Mas et al. (2018), Early and late changes in natural killer cells in response to ledipasvir/sofosbuvir treatment Hepatol Commun, 2(4):364-375.
PMID: 29619416
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Peppa et al. (2018), Adaptive Reconfiguration of Natural Killer Cells in HIV-1 Infection Front Immunol, 9(0):474.
PMID: 29616021
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Dishon et al. (2017), Inhibition of Myeloid Differentiation Factor 88 Reduces Human and Mouse T-Cell Interleukin-17 and IFN? Production and Ameliorates Experimental Autoimmune Encephalomyelitis Induced in Mice Front Immunol, 8(0):615.
PMID: 28611775
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L van den Dr et al. (2017), Immune activation in prolonged cART-suppressed HIV patients is comparable to that of healthy controls Virology, 509(0):133-139.
PMID: 28644978
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata J Immunol, 0(0).
PMID: 27412416
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Presnell S et al. (2015), Human natural killer cell microRNA: differential expression of MIR181A1B1 and MIR181A2B2 genes encoding identical mature microRNAs. Genes Immun, 16(1):89-98.
PMID: 25410655
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tsai C et al. (2015), Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus. J Immunol, 194(8):3890-900.
PMID: 25732728
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

de Groen R et al. (2015), IFN-lambda-mediated IL-12 production in macrophages induces IFN-gamma production in human NK cells. Eur J Immunol, 45(1):250-9.
PMID: 25316442
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mavropoulos A et al. (2014), Flow cytometric detection of p38 MAPK phosphorylation and intracellular cytokine expression in peripheral blood subpopulations from patients with autoimmune rheumatic diseases. J Immunol Res, 2014(0):671431.
PMID: 24741615
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gresnigt M et al. (2014), A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of Interleukin-1 receptor antagonist. PLoS Pathog, 10(3):e1003936.
PMID: 24603878
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Luetke-Eversloh M et al. (2014), NK cells gain higher IFN-gamma competence during terminal differentiation. Eur J Immunol, 44(7):2074-84.
PMID: 24752800
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sun M et al. (2014), The role of interleukin-1 receptor-associated kinases in Vogt-Koyanagi-Harada disease. PLoS ONE, 9(4):e93214.
PMID: 24690905
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hedl M et al. (2014), The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol, 192(12):5924-32.
PMID: 24842757
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Developmet

Jitprasertwong P et al. (2013), Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1. Cytokine, 65(2):222-30.
PMID: 24275551
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nielsen N et al. (2012), Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 7(2):e31959.
PMID: 22384114
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Le Garff-Tavernier M et al. (2011), Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies. Leukemia, 25(1):101-9.
PMID: 20975664
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhang H et al. (2011), IL-18 mediates proapoptotic signaling in renal tubular cells through a Fas ligand-dependent mechanism. Am. J. Physiol. Renal Physiol., 301(1):F171-8.
PMID: 21511702
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ghirelli C et al. (2010), Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood, 115(24):5037-40.
PMID: 20382843
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yu J et al. (2010), CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets. Blood, 115(2):274-81.
PMID: 19897577
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Beziat V et al. (2010), NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs. PLoS ONE, 5(8):e11966.
PMID: 20700504
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Beziat V et al. (2009), Fully functional NK cells after unrelated cord blood transplantation. Leukemia, 23(4):721-8.
PMID: 19151772
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Eid RE et al. (2009), Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 119(10):1424-32.
PMID: 19255340
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Das S et al. (2008), Japanese Encephalitis Virus infection induces IL-18 and IL-1beta in microglia and astrocytes: correlation with in vitro cytokine responsiveness of glial cells and subsequent neuronal death. J. Neuroimmunol., 195(1):60-72.
PMID: 18374991
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Vossen MT et al. (2008), CD27 defines phenotypically and functionally different human NK cell subsets. J. Immunol., 180(6):3739-45.
PMID: 18322179
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Pfleger C et al. (2007), Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 329(1):214-8.
PMID: 17964594
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Facco M et al. (2007), Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis. J. Leukoc. Biol., 82(4):946-55.
PMID: 17615381
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

de Matos CT et al. (2007), Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion. Immunology, 122(2):291-301.
PMID: 17521371
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ebert EC et al. (2006), Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 241(1):7-13.
PMID: 16914126
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Radstake TR et al. (2004), Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma. Arthritis Rheum., 50(12):3856-65.
PMID: 15593217
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kao CY et al. (2004), IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 173(5):3482-91.
PMID: 15322213
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Durali D et al. (2003), In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Blood, 102(12):4084-9.
PMID: 12893768

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Showing 1 - 10 of 39 citations Showing All

FAQs for Recombinant Human IL-18/IL-1F4 Protein

What is the difference between the two versions of Recombinant Human IL-18, Catalog # 9124-IL and Catalog # B001-5?

Catalog # 9124-IL is manufactured by R&D Systems, while Catalog # B001-5 is manufactured by MBL. Catalog # 9124-IL is lyophilized powder whereas Catalog # B001-5 is supplied in solution (PBS with BSA and sucrose). Activity of the two proteins is measured in similar cell-based assays; please consult the respective product-specific pages for assay details. Catalog # 9124-IL is competitively priced and has higher purity and lower endotoxin than Catalog # B001-5.

Recombinant Human IL-18/IL-1F4 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human IL-18/IL-1F4 Protein

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-18/IL-1F4

Citations for Recombinant Human IL-18/IL-1F4 Protein (39)

FAQs for Recombinant Human IL-18/IL-1F4 Protein

Product Documents for Recombinant Human IL-18/IL-1F4 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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