Recombinant Human IL-18/IL-1F4 Protein, CF

Name: Recombinant Human IL-18/IL-1F4 Protein, CF
Brand: R&D Systems
SKU: B003-5

R&D Systems, Inc. a Bio-Techne Brand

Catalog # B003-5

Citations (4)

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New Version Available - 9124-IL/CF

A New rh IL-18 is Available! Competitively priced; Higher purity; Lower endotoxin specifications; Made in-house!

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Source:	E. coli
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-18/IL-1F4 Protein, CF

Product Specifications for Recombinant Human IL-18/IL-1F4 Protein, CF

Source	E. coli-derived human IL-18/IL-1F4 protein 37-193 aa
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass	18 kDa
Activity	Induction of IFN-gamma by KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant human IL-18 was measured using human IFN-gamma ELISA. The activity of lot 111 was as follows: IL-18 final concentration 10 ng/mL; IFN-gamma induction 48.1 IU/mL IL-18 final concentration 20 ng/mL; IFN-gamma induction 64.3 IU/mL

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

B003-5

Formulation	25 μg in 250 μL volume of PBS containing 1% sucrose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8⁺ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4⁺ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

Item	Value
References	Dinarello, C.A. et al. (2013) Front. Immunol. 4:289. Smith, D.E. (2011) J. Leukoc. Biol. 89:383. Gu, Y. et al. (1997) Science 275:206. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Cheung, H. et al. (2005) J. Immunol. 174:5351. Novick, D. et al. (1999) Immunity 10:127. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769. Lalor, S.J. et al. (2011) J. Immunol. 186:5738. Li, J. et al. (2004) Cell. Immunol. 227:103. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665. Ushio, S. et al. (1996) J. Immunol. 156:4274. Gaggero, A. et al. (2004) Oncogene 23:7552. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Recombinant Human IL-18/IL-1F4 Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-18/IL-1F4 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Jamal Jame et al. (2021), Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 22(13).
PMID: 34281240
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

JW Choi et al. (2020), Proteome Analysis of Human Natural Killer Cell Derived Extracellular Vesicles for Identification of Anticancer Effectors Molecules, 25(21).
PMID: 33182448
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Gonzalez RM et al. (2008), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 7(6):2406-14.
PMID: 18422355
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Wu C et al. (2003), IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. J. Immunol., 170(11):5571-7.
PMID: 12759435

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Recombinant Human IL-18/IL-1F4 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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