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Human Total IL-18 DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

Human Total IL-18 / IL-1F4 ELISA Standard Curve
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DY318-05

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Key Product Details

Assay Type: Solid Phase Sandwich ELISA
Assay Range: 11.7 - 750 pg/mL
Sample Types: Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Total IL-18 DuoSet ELISA Features:
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Citations for Human Total IL-18 DuoSet ELISA (27)

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Customer Reviews for Human Total IL-18 DuoSet ELISA (10)

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  • Human Total IL-18 DuoSet ELISA
    Name: Andres Ocampo Carrillo
    Sample Tested: Cancer cell lysates
    Verified Customer | Posted 02/24/2023
    The kit was essential to quantify this cytokine in Caco-2 cells, previously grown for 21 days and then treated with pro-inflammatory factors (LPS and IFN-gamma). Results obtained agreed with other reports in the literature and were highly replicable.
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Supernates
    Verified Customer | Posted 02/14/2023
    HLF cells treated with LPS+ATP
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/05/2020
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Robert Berahovich
    Sample Tested: Tissue Culture Media
    Verified Customer | Posted 09/29/2020
    Detection of IL-18 produced by human CAR-T cells engineered to express IL-18 (PMC659), compared to normal CAR-T cells (PMC650).
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Eric Wang
    Sample Tested: Cell culture supernatant,THP-1 cells
    Verified Customer | Posted 06/11/2020
    The PMA-differentiated THP-1 cells were treated with or without LPS/ATP for 4 h. The production of IL-18 was tested from culture supernatants of treated THP-1 cells.
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant,THP-1 human acute monocytic leukemia cell line
    Verified Customer | Posted 11/13/2019
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Christian Cassel
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 10/02/2018
  • Human Total IL-18 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Bone marrow-derived dendritic cells
    Verified Customer | Posted 02/06/2018
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: BOCHATON Thomas
    Sample Tested: Serum
    Verified Customer | Posted 11/21/2017
    We used human serum at a dilution of 1:10. The result was very good.
    Human Total IL-18 DuoSet ELISA DY318-05
  • Human Total IL-18 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Human serum
    Verified Customer | Posted 05/17/2017
    Human Total IL-18 DuoSet ELISA DY318-05

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Product Documents for Human Total IL-18 DuoSet ELISA

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