wnvNS3 Protease Antibody (345712) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB29071CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Viral
Applications
Western Blot, Neutralization, Immunoprecipitation
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG3 Clone # 345712
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant wnvNS3 Protease protein
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079
Specificity
Detects West Nile NS3 Protease in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Applications for wnvNS3 Protease Antibody (345712) [CoraFluor™ 1]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: wnvNS3 Protease
Infection by mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication, and are therefore potential targets for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor.
Long Name
West Nile Virus NS3 Protease
Gene Symbol
POLY
Additional wnvNS3 Protease Products
Product Documents for wnvNS3 Protease Antibody (345712) [CoraFluor™ 1]
Product Specific Notices for wnvNS3 Protease Antibody (345712) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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