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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2A Clone # 741307

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3F
Ala19 Pro775 (Arg583Ala and Arg586Ala)
Accession # O88632

Specificity

Detects mouse Semaphorin 3F in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 3A, 3B, 3C, 3E, 4C, 4D, 4G, recombinant human Semaphorin 3D or 3F is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Semaphorin 3F Antibody (741307) [CoraFluor™ 1]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Semaphorin 3F

The Semaphorins make up the largest family of axon guidance cues yet described. Semaphorins are divided into 8 classes (classes 3-7 found in vertebrates). Class 3 Semaphorins are secreted, classes 4 through 6 are transmembrane proteins, and class 7 are membrane associated via glycosylphosphatidylinositol (GPI) linkage. They are characterized structurally by a conserved ~400 amino acid sema domain. They are classically described as collapsing factors and mediators of axon repulsion, although they may also act as context-dependent chemoattractants. Semaphorins have been shown to have roles in cardiovascular development and in the regulation of immune cell antigen presentation. Receptors or receptor complexes that mediate semaphorin signaling include proteins of the Neuropilin and Plexin families.

Alternate Names

Sema3F, SEMA4, SEMAK

Gene Symbol

SEMA3F

Additional Semaphorin 3F Products

Product Documents for Semaphorin 3F Antibody (741307) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Semaphorin 3F Antibody (741307) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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