Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?

Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

S100A9 Antibody (MAC387) [CoraFluor™ 1]

Name: S100A9 Antibody (MAC387) [CoraFluor™ 1]
Brand: Novus Biologicals
SKU: NBP2-47667CL1

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47667CL1

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Product Datasheet / COA / SDS

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Conjugate

Catalog #

Unconjugated

NBP2-45295

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Alexa Fluor 350

NBP2-47667AF350

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Alexa Fluor 405

NBP2-47667AF405

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Alexa Fluor 488

NBP2-47667AF488

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Alexa Fluor 532

NBP2-47667AF532

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Alexa Fluor 594

NBP2-47667AF594

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Alexa Fluor 647

NBP2-47667AF647

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Alexa Fluor 700

NBP2-47667AF700

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Alexa Fluor 750

NBP2-47667AF750

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Allophycocyanin

NBP2-47667APC

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Biotin

NBP2-47667B

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DyLight 350

NBP2-47667UV

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DyLight 405

NBP2-47667V

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DyLight 488

NBP2-47667G

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DyLight 550

NBP2-47667R

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DyLight 594

NBP2-47667DL594

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DyLight 650

NBP2-47667C

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DyLight 680

NBP2-47667FR

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DyLight 755

NBP2-47667IR

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FITC

NBP2-47667F

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HRP

NBP2-47667H

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Janelia Fluor 525

NBP2-47667JF525

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Janelia Fluor 549

NBP2-47667JF549

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Janelia Fluor 646

NBP2-47667JF646

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Janelia Fluor 669

NBP2-47667JF669

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mFluor Violet 450 SE

NBP2-47667MFV450

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mFluor Violet 500 SE

NBP2-47667MFV500

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mFluor Violet 610 SE

NBP2-47667MFV610

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NBP2-47667PE

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PerCP

NBP2-47667PCP

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Forumulation

Catalog #

Azide and BSA Free

NBP2-47667

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Equine, Feline, Goat, Guinea Pig, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # MAC387

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Affinity Purified monocyte membrane preparation

Localization

Cytoplasmic

Specificity

Recognizes the L1 or Calprotectin molecule (S100A9), an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. This monoclonal antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for S100A9 Antibody (MAC387) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.