Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 474915

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PGC1 alpha Antibodies »

Product Specifications

Immunogen

E. coli-derived human PGC1 alpha
Glu11-Ile280
Accession # Q9UBK2

Specificity

Detects human PGC1 alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for PGC1 alpha Antibody (474915) [CoraFluor™ 1]

PGC1 alpha Antibody (474915) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for PGC1 alpha Antibody (474915) [CoraFluor™ 1]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB10784. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: PGC1 alpha

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Long Name

Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha

Alternate Names

LEM6, PPARGC1A

Additional PGC1 alpha Products

Product Documents for PGC1 alpha Antibody (474915) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PGC1 alpha Antibody (474915) [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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