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Myosin Heavy Chain Antibody Best Seller

Catalog # MAB4470 | R&D Systems, Inc. a Bio-Techne Brand
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MAB4470
MAB4470-SP

Key Product Details

Species Reactivity

Multi-Species

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MF20

Product Summary for Myosin Heavy Chain Antibody

Immunogen

Chicken pectoralis-derived Myosin

Specificity

Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Myosin Heavy Chain Antibody

Myosin Heavy Chain antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Myosin Heavy Chain in C2C12 Mouse Cell Line.

Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myosin Heavy Chain antibody in Human Skeletal Muscle by Immunohistochemistry (IHC-P).

Myosin Heavy Chain in Human Skeletal Muscle.

Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr).

Myosin Heavy Chain in Mouse Skeletal Muscle.

Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Myosin Heavy Chain Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle and perfusion fixed frozen sections of mouse skeletal muscle

Western Blot

Stains, C.I., et al. (2012) Chem Biol. 19:210.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 9 reviews rated 4.6 using MAB4470 in the following applications:

Published Applications

Read 95 publications using MAB4470 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myosin Heavy Chain

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Alternate Names

MYHC

Product Documents for Myosin Heavy Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Myosin Heavy Chain Antibody

For research use only

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FAQs for Myosin Heavy Chain Antibody

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  • Q: Does Myosin Heavy Chain Antibody, Catalog # MAB4470, detect Myosin Heavy Chain in all mammalian species?

    A: Myosin Heavy Chain is a highly conserved protein sequence. Although we have not tested every mammalian species, we expect it will bind to Myosin Heavy Chain in all mammalian species.

Showing  1 - 1 of 1 FAQ Showing All

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