Mouse Gas6 Antibody

Gas6 in Mouse Intestine.

Gas6 was detected in perfusion fixed paraffin-embedded sections of mouse intestine using Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Gas6 by Immunohistochemistry

Gas6 & Axl expression in the cecal mucosa during K. pneumoniae infection decreases with aging.(A & B) Sections of cecal mucosa (A) or liver (B) were from mice aged 15 or 57 weeks at 2 days after infection with K. pneumoniae ATCC43816 pmCherry & immunostained with an anti-Gas6 antibody & an anti-Axl antibody. Each image has six independent replicates. Scale bar = 50 μm. (C) Detection of Axl, Gas6, ZO-1, & occludin by WBting. Cecum & liver tissues were collected, homogenized, & analyzed using an anti-Axl antibody, an anti-Gas6 antibody, an anti-ZO-1 antibody, or an anti-occludin antibody. Each lane of WBting images represents the protein from an individual mouse (mouse number = 5 (cecum) or 4 (liver) per group). Western blotting analysis represents three independent replicates. (D) Western blotting signal intensities were analyzed by ImageJ software. Each dot represents the value collected from an individual mouse (mouse number = 5 (cecum) or 4 (liver) per group). Data are presented as the mean ± SD. NS: not significant, *p < 0.05, **p < 0.01. p values were calculated by the Student’s t test. (E) Linear correlation between Gas6 expression & Axl expression in the cecum or liver of mice aged 15 (red circles) or 57 (blue circles) weeks infected with K. pneumoniae ATCC43816 pmCherry. Each dot represents an ELISA measured value of each protein sample collected from an individual mouse (mouse number = 5 per group). r > 0.70 & p < 0.05. (F) The population of CD11b+F4/80+ macrophages in the intestinal mucosa of young (14-week-old) & old (56-week-old) mice was examined by staining with anti-F4/80 & anti-CD11b antibodies. The percentage of F4/80-positive/CX3CR1-negative cells in 14-week-old & 56-week-old mice was 29.5% & 8.87%, respectively. Data are representative of four mice per group. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37289655), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Gas6 by Western Blot

Gas6 & Axl expression in the cecal mucosa during K. pneumoniae infection decreases with aging.(A & B) Sections of cecal mucosa (A) or liver (B) were from mice aged 15 or 57 weeks at 2 days after infection with K. pneumoniae ATCC43816 pmCherry & immunostained with an anti-Gas6 antibody & an anti-Axl antibody. Each image has six independent replicates. Scale bar = 50 μm. (C) Detection of Axl, Gas6, ZO-1, & occludin by WBting. Cecum & liver tissues were collected, homogenized, & analyzed using an anti-Axl antibody, an anti-Gas6 antibody, an anti-ZO-1 antibody, or an anti-occludin antibody. Each lane of WBting images represents the protein from an individual mouse (mouse number = 5 (cecum) or 4 (liver) per group). Western blotting analysis represents three independent replicates. (D) Western blotting signal intensities were analyzed by ImageJ software. Each dot represents the value collected from an individual mouse (mouse number = 5 (cecum) or 4 (liver) per group). Data are presented as the mean ± SD. NS: not significant, *p < 0.05, **p < 0.01. p values were calculated by the Student’s t test. (E) Linear correlation between Gas6 expression & Axl expression in the cecum or liver of mice aged 15 (red circles) or 57 (blue circles) weeks infected with K. pneumoniae ATCC43816 pmCherry. Each dot represents an ELISA measured value of each protein sample collected from an individual mouse (mouse number = 5 per group). r > 0.70 & p < 0.05. (F) The population of CD11b+F4/80+ macrophages in the intestinal mucosa of young (14-week-old) & old (56-week-old) mice was examined by staining with anti-F4/80 & anti-CD11b antibodies. The percentage of F4/80-positive/CX3CR1-negative cells in 14-week-old & 56-week-old mice was 29.5% & 8.87%, respectively. Data are representative of four mice per group. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37289655), licensed under a CC-BY license. Not internally tested by R&D Systems.