Mouse ACE‑2 Antibody

Catalog # AF3437 | R&D Systems, Inc. a Bio-Techne Brand
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AF3437
AF3437-SP
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse ACE‑2 Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740
Accession # Q8R0I0

Specificity

Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ACE‑2 Antibody

Detection of Mouse ACE-2 antibody by Western Blot.

Detection of Mouse ACE‑2 by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ACE-2 antibody in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP antibody by Flow Cytometry.

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
ACE‑2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

ACE‑2 in Mouse Kidney.

ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Applications for Mouse ACE‑2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse ACE-2 DuoSet ELISA Kit (Catalog # DY3437-05) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse ACE-2 and eGFP

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ACE‑2 (Catalog # 3437‑ZN), see our available Western blot detection antibodies

Simple Western

2.5 µg/mL
Sample: Mouse kidney tissue

Western Blot

0.25 µg/mL
Sample: Mouse kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using AF3437 in the following applications:

Published Applications

Read 9 publications using AF3437 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

References

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.

Long Name

Angiotensin I Converting Enzyme 2

Alternate Names

ACE2, ACEH

Entrez Gene IDs

480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 59272 (Human); 108390919 (Malayan Pangolin); 70008 (Mouse); 100144303 (Porcine); 302668 (Rat)

Gene Symbol

ACE2

UniProt

Q8R0I0

Product Documents for Mouse ACE‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ACE‑2 Antibody

For research use only

Citations for Mouse ACE‑2 Antibody (9)

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Customer Reviews for Mouse ACE‑2 Antibody (3)

4.7 out of 5
3 customer ratings
5 stars
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4 stars
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  • Mouse ACE-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart tissue
    Species: Mouse
    Verified Customer | Posted 08/26/2016
    Mouse ACE‑2 Antibody AF3437
  • Mouse ACE-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult kidney
    Species: Mouse
    Verified Customer | Posted 06/10/2016
    Mouse ACE‑2 Antibody AF3437
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22777933
    Species: Mouse
    Verified Customer | Posted 01/07/2015

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