MEK1/2 [p Ser226, p Ser222, p Ser222, p Ser218] Antibody (1020E) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB8407CL1
Recombinant Monoclonal Antibody.
![MEK1/2 [p Ser226, p Ser222, p Ser222, p Ser218] Antibody (1020E) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab8407cl1_mek1-2-p-ser226-p-ser222-p-ser222-p-ser218-mab-corafluor-1-299202515272221.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Rabbit IgG Clone # 1020E
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phosphopeptide containing the human, mouse, and rat MEK1 S218/S222 site
Modification
p Ser226
Specificity
Detects human, mouse, and rat MEK1 and MEK2 dually phosphorylated at S218/S222 and S222/S226, respectively.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: MEK1/MEK2
MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.
Long Name
Mitogen-activated Protein Kinase Kinase 1
Alternate Names
CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Symbol
MAP2K1
Additional MEK1/MEK2 Products
Product Specific Notices
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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