IL-12/IL-35 p35 Antibody (116219) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1171CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 116219
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant porcine IL-12 p35/p40 heterodimer
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053
p40 Ile23-Asn324, p35 Arg26-Ser222
Accession # Q28938, Q29053
Specificity
Detects the heterodimeric form of porcine IL‑12 (p35/p40) in direct ELISAs. Western blotting data indicates recognition of the IL-12/IL-35 p35 subunit. In direct ELISAs and Western blots, 20‑100% cross-reactivity with the disulfide‑linked heterodomeric forms of recombinant mouse IL‑12 and recombinant human IL-12 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Applications for IL-12/IL-35 p35 Antibody (116219) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: IL-12/IL-35 p35
Long Name
Interleukin 12 p35
Alternate Names
IL-12A, IL35p35
Additional IL-12/IL-35 p35 Products
Product Documents for IL-12/IL-35 p35 Antibody (116219) [CoraFluor™ 1]
Product Specific Notices for IL-12/IL-35 p35 Antibody (116219) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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