Human/Mouse/Rat NKX2.2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8167
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunocytochemistry, Bioassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 883411
Product Specifications
Immunogen
E. coli-derived recombinant human NKX2.2
Met1-Lys128
Accession # O95096
Met1-Lys128
Accession # O95096
Specificity
Detects human NKX2.2 in ELISA and Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat NKX2.2 Antibody
Detection of Human and Mouse NKX2.2 by Western Blot.
Western blot shows lysates of A-673 human Ewing's sarcoma cell line and betaTC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NKX2.2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NKX2.2 in A673 Human Cell Line.
NKX2.2 was detected in immersion fixed A673 human Ewing sarcoma cell line using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
NKX2.2 in Rat Cortical Stem Cells.
NKX2.2 was detected in immersion fixed rat cortical stem cells differentiated for 7 days using Mouse Anti-Human/Mouse/Rat NKX2.2 Monoclonal Antibody (Catalog # MAB8167) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse/Rat NKX2.2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A637 human Ewing sarcoma cell line and rat cortical stem cells differentiated for 7 days
Sample: Immersion fixed A637 human Ewing sarcoma cell line and rat cortical stem cells differentiated for 7 days
Western Blot
2 µg/mL
Sample: A‑673 human Ewing's sarcoma cell line and betaTC‑6 mouse beta cell insulinoma cell line
Sample: A‑673 human Ewing's sarcoma cell line and betaTC‑6 mouse beta cell insulinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKX2.2
Long Name
NK2 Homeobox 2
Alternate Names
NKX2B
Gene Symbol
NKX2-2
UniProt
Additional NKX2.2 Products
Product Documents for Human/Mouse/Rat NKX2.2 Antibody
Product Specific Notices for Human/Mouse/Rat NKX2.2 Antibody
For research use only
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