Human IL‑29/IFN-lambda 1 Antibody

Name: Human IL‑29/IFN-lambda 1 Antibody
Brand: R&D Systems
SKU: MAB15981
Rating: 5 (2 reviews)

Catalog # MAB15981 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (10)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 247801

Product Summary for Human IL‑29/IFN-lambda 1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-29/IFN-lambda 1
Gly20-Thr200
Accession # Q8IU54

Specificity

Detects human IL-29/IFN-lambda 1 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) IL-28B/IFN-lambda 3 and 20% with rhIL‑28A is observed. No cross-reactivity or interference was observed with rhIFN-alpha A, rhIFN-alpha B2, rhIFN-alpha G, rhIFN-alpha I, or rhIFN‑ beta1A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human IL‑29/IFN-lambda 1 Antibody

IL‑29/IFN-lambda 1 Inhibition of EMCV‑induced Cytopathy and Neutralization by Human IL‑29/IFN-lambda 1 Antibody.

Recombinant Human IL-29/IFN-lambda 1 (Catalog # 1598-IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-29/IFN-lambda 1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anri-Human IL-29/IFN-lambda 1 Monoclonal Antibody (Catalog # MAB15981). The ND₅₀ is typically 1-4 µg/mL.

Applications for Human IL‑29/IFN-lambda 1 Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑29/IFN-lambda 1 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑29/IFN-lambda 1.

IL-29/IFN-lambda 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IL‑29/IFN-lambda 1 Biotinylated Antibody (Catalog # BAF1598)
Standard: Recombinant Human IL-29/IFN-lambda 1 Protein (Catalog # 1598-IL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-29/IFN-lambda 1

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% aa sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.

References

Vilcek, J. (2003) Nature Immunol. 4:8.
Sheppard, P. et al. (2003) Nature Immunol. 4:63.
Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Entrez Gene IDs

282618 (Human)

Gene Symbol

IFNL1

UniProt

Q8IU54

Product Documents for Human IL‑29/IFN-lambda 1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL‑29/IFN-lambda 1 Antibody

For research use only

Citations
Reviews

Citations for Human IL‑29/IFN-lambda 1 Antibody (10)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL‑29/IFN-lambda 1 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Essaidi-La et al. (2020-06-24), Interferon-Dependent and Respiratory Virus-Specific Interference in Dual Infections of Airway Epithelia Sci Rep, 2020-010(1):10246.
PMID: 32581261
Species: Human
Sample Types: Plasma
Applications: ELISA Capture

V Oke et al. (2019-04-29), High levels of circulating interferons type I, type II and type III associate with distinct clinical features of active systemic lupus erythematosus Arthritis Res. Ther., 2019-021(1):107.
PMID: 31036046
Species: Human
Sample Types: Serum

V Oke et al. (2017-06-15), IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE) Arthritis Res. Ther., 2017-019(1):139.
PMID: 28619037
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture)

Iva Gunnarsson (2016-11-22), Interferon (IFN)-? is a potential mediator in lupus nephritis Lupus Sci Med, 2016-13(1):e000170.
PMID: 27933198
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Villenave R et al. (2015-09-30), Induction and Antagonism of Antiviral Responses in Respiratory Syncytial Virus-Infected Pediatric Airway Epithelium. J Virol, 2015-089(24):12309-18.
PMID: 26423940
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Persson B et al. (2014-07-17), Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation. PLoS ONE, 2014-09(7):e102368.
PMID: 25033192
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Brownell J et al. (2013-11-20), Direct, interferon-independent activation of the CXCL10 promoter by NF-kappaB and interferon regulatory factor 3 during hepatitis C virus infection. J Virol, 2013-188(3):1582-90.
PMID: 24257594
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Brownell J et al. (2013-06-12), Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes. J Hepatol, 2013-059(4):701-8.
PMID: 23770038
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Stone et al. (2013-04-18), Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. PLoS Pathog, 2013-09(4):e1003316.
PMID: 23637605
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Yin Z et al. (2012-08-13), Type III IFNs are produced by and stimulate human plasmacytoid dendritic cells. J Immunol, 2012-0189(6):2735-45.
PMID: 22891284

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