Recombinant Human IL-29/IFN-lambda 1 Protein Best Seller

Name: Recombinant Human IL-29/IFN-lambda 1 Protein
Brand: R&D Systems
SKU: 1598-IL
Rating: 4 (3 reviews)

Catalog # 1598-IL | R&D Systems, Inc. a Bio-Techne Brand

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Citations (42)

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(NS0-expressed)

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Key Product Details

Source:	NS0
Accession #:	Q8IU54
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-29/IFN-lambda 1 Protein

Product Specifications for Recombinant Human IL-29/IFN-lambda 1 Protein

Source	Mouse myeloma cell line, NS0-derived human IL-29/IFN-lambda 1 protein Gly20-Thr200, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly20
Predicted Molecular Mass	21.4 kDa
SDS-PAGE	26-35 kDa, reducing conditions
Activity	Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED₅₀ for this effect is 1-5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1598-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1598-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-29/IFN-lambda 1

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL‑28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.

Item	Value
References	Vilcek, J. (2003) Nature Immunol. 4:8. Sheppard, P. et al. (2003) Nature Immunol. 4:63. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Long Name	Interleukin 29
Entrez Gene IDs	282618 (Human)
Alternate Names	IFN-lambda 1, IFN-lambda-1, IFNL1, IL-29, IL29, ZCYTO21, cytokine Zcyto21, interferon lambda-1, interferon, lambda 1, interleukin 29 (interferon, lambda 1), interleukin-29

Citations for Recombinant Human IL-29/IFN-lambda 1 Protein (42)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-29/IFN-lambda 1 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Tsai et al. (2022-11-13), The E3 ligase subunit FBXO45 binds the interferon-lambda receptor and promotes its degradation during influenza virus infection The Journal of Biological Chemistry, 2022-10(0):102698.
PMID: 36379255
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Fedoriw et al. (2022-04-01), Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response Cancer Immunology Research, 2022-00(0).
PMID: 35181787
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Das et al. (2022-01-15), Astrocyte Control of Zika Infection Is Independent of Interferon Type I and Type III Expression Biology, 2022-011(1).
PMID: 35053142
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CF Hatton et al. (2021-12-07), Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2 Nature Communications, 2021-112(1):7092.
PMID: 34876592
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EA Monson et al. (2021-07-14), Intracellular lipid droplet accumulation occurs early following viral infection and is required for an efficient interferon response Nature Communications, 2021-012(1):4303.
PMID: 34262037
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Medaglia et al. (2021-06-01), Interferon Lambda Delays the Emergence of Influenza Virus Resistance to Oseltamivir Microorganisms, 2021-09(6).
PMID: 34205874
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HC Hawerkamp et al. (2021-05-21), Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation Arthritis Research Therapy, 2021-05-21;23(1):144.
PMID: 34020693
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Hayn et al. (2021-04-27), Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities Cell Reports, 2021-035(7):109126.
PMID: 33974846
Species: Primate - C. aethiops
Sample Types: Whole Cells
Applications: Bioassay

X Zhao et al. (2021-02-12), Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus Stem Cell Reports, 2021-016(3):493-504.
PMID: 33626333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Syedbasha et al. (2020-10-06), Interferon-&lambda Enhances the Differentiation of Naive B Cells into Plasmablasts via the mTORC1 Pathway Cell Rep, 2020-133(1):108211.
PMID: 33027651
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CJ Stairiker et al. (2020-07-17), Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells PLoS ONE, 2020-015(7):e0236195.
PMID: 32678841
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Essaidi-La et al. (2020-06-24), Interferon-Dependent and Respiratory Virus-Specific Interference in Dual Infections of Airway Epithelia Sci Rep, 2020-010(1):10246.
PMID: 32581261
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

Y Chen et al. (2020-06-02), Dendritic cells-derived interferon-lambda1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis Cell Death Dis, 2020-011(6):414.
PMID: 32488049
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

AM Kell et al. (2020-04-24), RIG-I-like receptor activation drives type I IFN and antiviral signaling to limitï¿½Hantaan orthohantavirus replication PLoS Pathog., 2020-016(4):e1008483.
PMID: 32330200
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JH Chung et al. (2019-08-31), Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency Cytokine, 2019-0125(0):154833.
PMID: 31479875
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Good et al. (2019-03-06), Type III interferon signaling restricts enterovirus 71 infection of goblet cells Sci Adv, 2019-05(3):eaau4255.
PMID: 30854425
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Xu et al. (2019-02-08), MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway Elife, 2019-08(0).
PMID: 30735121
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

I Angelidou et al. (2018-04-30), REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1? Inflammatory Response in Active Ulcerative Colitis J. Immunol., 2018-00(0).
PMID: 29712770
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Cell Culture

SS Kwek et al. (2018-03-12), A systematic approach to the development of a safe live attenuated Zika vaccine Nat Commun, 2018-09(1):1031.
PMID: 29531213
Species: Human
Sample Types: Whole Cells
Applications: Stimulation

A Pierangeli et al. (2018-03-10), Interferon lambda receptor 1 (IFNL1R) transcript is highly expressed in rhinovirus bronchiolitis and correlates with disease severity J. Clin. Virol., 2018-0102(0):101-109.
PMID: 29549834
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AA Obajemu et al. (2017-10-25), IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling J. Immunol., 2017-10(0).
PMID: 29070670
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Corry et al. (2017-08-07), Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface Proc. Natl. Acad. Sci. U.S.A., 2017-00(0).
PMID: 28784796
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

NA Ilyushina et al. (2017-07-27), Generation and characterization of interferon-lambda 1-resistant H1N1 influenza A viruses PLoS ONE, 2017-012(7):e0181999.
PMID: 28750037
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PS Sung et al. (2017-06-19), IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection Sci Rep, 2017-07(1):3821.
PMID: 28630501
Species: Human
Sample Types: Serum
Applications: ELISA (Standard)

V Oke et al. (2017-06-15), IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE) Arthritis Res. Ther., 2017-019(1):139.
PMID: 28619037
Species: Human
Sample Types: Serum
Applications: ELISA (Standard)

Iva Gunnarsson (2016-11-22), Interferon (IFN)-? is a potential mediator in lupus nephritis Lupus Sci Med, 2016-13(1):e000170.
PMID: 27933198
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-11-14), Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling Nat. Med., 2016-10(0).
PMID: 27841874
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-07-26), Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016-06(0):24530.
PMID: 27456172
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bordi L et al. (2015-02-18), Antagonistic antiviral activity between IFN-lambda and IFN-alpha against lethal Crimean-Congo hemorrhagic fever virus in vitro. PLoS ONE, 2015-010(2):e0116816.
PMID: 25693189
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ding S et al. (2014-01-07), Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth. PLoS Biol, 2014-012(1):e1001758.
PMID: 24409098
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cao Y et al. (2013-12-11), IL-27, a cytokine, and IFN-lambda1, a type III IFN, are coordinated to regulate virus replication through type I IFN. J Immunol, 2013-1192(2):691-703.
PMID: 24337382
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Control

Popugaeva E et al. (2012-04-24), Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE, 2012-07(4):e35587.
PMID: 22545121
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Li Q et al. (2011-09-27), Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-lambda genes produced anti-tumour effects. Br. J. Cancer, 2011-0105(9):1302-12.
PMID: 21952623
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zahn S et al. (2010-08-19), Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus. J. Invest. Dermatol., 2010-0131(1):133-40.
PMID: 20720564
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Witte K et al. (2009-10-01), Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Genes Immun., 2009-110(8):702-14.
PMID: 19798076
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Duluc D et al. (2009-07-15), Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 2009-0125(2):367-73.
PMID: 19378341
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bin L et al. (2009-07-03), Inhibition of S100A11 gene expression impairs keratinocyte response against vaccinia virus through downregulation of the IL-10 receptor 2 chain. J. Allergy Clin. Immunol., 2009-0124(2):270-7, 277.e1.
PMID: 19577285
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kato A et al. (2008-04-14), Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J. Allergy Clin. Immunol., 2008-0121(6):1385-92, 1392.
PMID: 18410958
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wolk K et al. (2008-02-15), Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes. J. Leukoc. Biol., 2008-083(5):1181-93.
PMID: 18281438
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kato A et al. (2007-07-15), TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells. J. Immunol., 2007-0179(2):1080-7.
PMID: 17617600
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Meager A et al. (2005-07-21), Biological activity of interleukins-28 and -29: comparison with type I interferons. Cytokine, 2005-031(2):109-18.
PMID: 15899585

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Name: Anonymous

Application: CellProlif

Verified Customer | Posted 12/03/2018
Recombinant Human IL-29/IFN-lambda 1 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 06/01/2018

The recombinant IFN-lambda served as the calibration material in an ELISA which used MAB15981 and BAF1598 as matched pair antibodies.
Recombinant Human IL-29/IFN-lambda 1 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/01/2017

Recombinant Human IL-29/IFN-lambda 1 Protein Best Seller

Catalog # 1598-IL | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-29/IFN-lambda 1 Protein

Formulation, Preparation and Storage

Carrier Free

1598-IL

Carrier

1598-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-29/IFN-lambda 1

Citations for Recombinant Human IL-29/IFN-lambda 1 Protein (42)

Customer Reviews for Recombinant Human IL-29/IFN-lambda 1 Protein (3)

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Recombinant Human IL-29/IFN-lambda 1 Protein

Recombinant Human IL-29/IFN-lambda 1 Protein

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