HEK293 human embryonic kidney cell line transfected with either (A) Human GPRC5D or (B) irrelevant protein, and eGFP was stained with Mouse anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB63001) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human GPRC5D Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Long Name

G Protein-coupled Receptor, Family D, Group 5, Member D

Alternate Names

G protein-coupled receptor, family C, group 5, member D, G-protein coupled receptor family C group 5 member D, MGC129713, MGC129714, orphan G-protein coupled receptor

Entrez Gene IDs

55507 (Human); 93746 (Mouse); 500349 (Rat)

Gene Symbol

GPRC5D

UniProt

Q9NZD1 (Human)

Additional GPRC5D Products

Product Documents for Human GPRC5D Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPRC5D Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPRC5D Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB63001

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPRC5D Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPRC5D Antibody

Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry.