HEK293 human embryonic kidney cell line transfected with (A) Human GPRC5A or (B) irrelevant protein and eGFP, was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of GPRC5A in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human GPRC5A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human GPRC5A and eGFP

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member A

Alternate Names

RAI3, RAIG1

Entrez Gene IDs

9052 (Human); 232431 (Mouse); 312790 (Rat)

Gene Symbol

GPRC5A

UniProt

Q8NFJ5

Additional GPRC5A Products

Product Documents for Human GPRC5A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPRC5A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPRC5A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5239

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all GPRC5A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPRC5A Antibody

Detection of GPRC5A in hGPRC5A HEK293/eGFP transfectants by Flow Cytometry.