Human DAP12 Antibody
Bio-Techne includes R&D Systems | Catalog # MAB5240
Discontinued Product
MAB5240 has been discontinued and is replaced by
MAB52401.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
CyTof, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 406288
Product Summary for Human DAP12 Antibody
Immunogen
Human Dap12 synthetic peptide
QGQRSDVYSDLNTQRPYYK
Accession # O43914
QGQRSDVYSDLNTQRPYYK
Accession # O43914
Specificity
Detects human DAP12 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DAP12 Antibody
Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry.
Human peripheral blood CD56+natural killer cells were stained with Human DAP12 Monoclonal Antibody (Catalog # MAB5240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human DAP12 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line, mock transfected, and primary human CD56+NK cells. PVDF membrane was probed with 5 µg/mL of Human DAP12 Monoclonal Antibody (Catalog # MAB5240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAP12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human DAP12 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood CD56+ natural killer cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human peripheral blood CD56+ natural killer cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
5 µg/mL
Sample: primary human CD56+ NK cell lysates under reducing conditions only
Sample: primary human CD56+ NK cell lysates under reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DAP12
DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.
Long Name
DNAX-activation Protein 12
Alternate Names
KARAP, TYROBP
Gene Symbol
TYROBP
UniProt
Additional DAP12 Products
Product Documents for Human DAP12 Antibody
Product Specific Notices for Human DAP12 Antibody
For research use only
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