Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14658CL1
![Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/nbp3-14658cl1_mouse-human-coronavirus-spike-protein-mab-29-cl1-25920251329924.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
HCoV-OC43
Applications
Western Blot, ELISA
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 29
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein.
Specificity
Recognizes the Spike of HCoV-OC43. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-229E, HCoV-HKU1 (isolate N5).Has cross-reactivity in ELISA and WB with:HCoV-OC43 Spike S1+S2 ECD-His No cross-reactivity in ELISA and WB with:SARS-CoV-2 Spike S1-His SARS-CoV-2 Spike S1 NTD-Fc & AVI SARS-CoV-2 Spike S1 NTD-His & AVI SARS-CoV-2 Spike RBD-His SARS-CoV-2 Spike S1+S2 ECD-His SARS-CoV-2 Spike S2 ECD-His SARS-CoV Spike S1+S2 ECD-His MERS-CoV Spike S1+S2 ECD-His HCoV-229E Spike S1+S2 ECD-His HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His HCoV-NL63 Spike S1+S2 ECD-His
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Human Coronavirus Spike Protein
Alternate Names
HKU1, Spike glycoprotein
Gene Symbol
S
Additional Human Coronavirus Spike Protein Products
Product Documents for Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]
Product Specific Notices for Human Coronavirus Spike Protein Antibody (29) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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