Human Coronavirus Spike Protein Antibody (29) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14658
![Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658]](https://resources.bio-techne.com/images/products/Human-Coronavirus-Spike-Protein-Antibody-29-Western-Blot-NBP3-14658-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
HCoV-OC43
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 29
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein.
Specificity
Recognizes the Spike of HCoV-OC43. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-229E, HCoV-HKU1 (isolate N5).Has cross-reactivity in ELISA and WB with:HCoV-OC43 Spike S1+S2 ECD-His No cross-reactivity in ELISA and WB with:SARS-CoV-2 Spike S1-His SARS-CoV-2 Spike S1 NTD-Fc & AVI SARS-CoV-2 Spike S1 NTD-His & AVI SARS-CoV-2 Spike RBD-His SARS-CoV-2 Spike S1+S2 ECD-His SARS-CoV-2 Spike S2 ECD-His SARS-CoV Spike S1+S2 ECD-His MERS-CoV Spike S1+S2 ECD-His HCoV-229E Spike S1+S2 ECD-His HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His HCoV-NL63 Spike S1+S2 ECD-His
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Human Coronavirus Spike Protein Antibody (29) - Azide and BSA Free
Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658]
Western Blot: Human Coronavirus Spike Protein Antibody (29) [NBP3-14658] - Anti-HCoV-OC43 mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane 1: SARS-CoV-2 Spike S1-His Lane 2: SARS-CoV-2 Spike S1 NTD-Fc & AVILane 3: SARS-CoV-2 Spike S1 NTD-His & AVILane 4: SARS-CoV-2 Spike RBD-HisLane 5: SARS-CoV-2 Spike S2 ECD-HisLane 6: SARS-CoV-2 Spike S1+S2 ECD-HisLane 7: SARS-CoV Spike S1+S2 ECD-HisLane 8: MERS-CoV Spike S1+S2 ECD-HisLane 9: HCoV-NL63 Spike S1+S2 ECD-HisLane 10: HCoV-229E Spike S1+S2 ECD-HisLane 11: HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-HisLane 12: HCoV-OC43 Spike S1+S2 ECD-HisSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Applications for Human Coronavirus Spike Protein Antibody (29) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Western Blot
1:1000-1:5000
Application Notes
Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Human Coronavirus Spike Protein
Alternate Names
HKU1, Spike glycoprotein
Gene Symbol
S
Additional Human Coronavirus Spike Protein Products
Product Documents for Human Coronavirus Spike Protein Antibody (29) - Azide and BSA Free
Product Specific Notices for Human Coronavirus Spike Protein Antibody (29) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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