Human Coronavirus Spike Protein Antibody (07) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14665CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
HCoV-HKU1
Applications
ELISA
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 07
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Coronavirus Spike S1+S2 ECD protein.
Specificity
Recognizes the Spike of HCoV-HKU1 (isolate N5). Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-229E, HCoV-OC43.Has cross-reactivity in ELISA with:HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His No cross-reactivity in ELISA with:SARS-CoV-2 Spike S1-His SARS-CoV-2 Spike S1 NTD-Fc & AVI SARS-CoV-2 Spike S1 NTD-His & AVI SARS-CoV-2 Spike RBD-His SARS-CoV-2 Spike S1+S2 ECD-His SARS-CoV-2 Spike S2 ECD-His SARS-CoV Spike S1+S2 ECD-His MERS-CoV Spike S1+S2 ECD-His HCoV-NL63 Spike S1+S2 ECD-His HCoV-229E Spike S1+S2 ECD-His HCoV-OC43 Spike S1+S2 ECD-His
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Human Coronavirus Spike Protein Antibody (07) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for Human Coronavirus Spike Protein Antibody (07) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP3-14665. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Human Coronavirus Spike Protein
Alternate Names
HKU1, Spike glycoprotein
Gene Symbol
S
Additional Human Coronavirus Spike Protein Products
Product Documents for Human Coronavirus Spike Protein Antibody (07) [CoraFluor™ 1]
Product Specific Notices for Human Coronavirus Spike Protein Antibody (07) [CoraFluor™ 1]
Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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