Human Coagulation Factor III/Tissue Factor Antibody

Detection of Coagulation Factor III/Tissue Factor by Western Blot

Mechanistic target of rapamycin (mTOR) inhibitor differentially targets TF-mRNA transcription or TF protein degradation in a cellular context-dependent manner. (A,B) U87MG and HCC827 (A), H1975 and PC9 (B) cells were treated as indicated, subjected to qPCR analysis of TF mRNA as described in section “Materials and Methods.” The relative mRNA levels are shown. (C,D) H1975 (C) and PC9 (D) cells were treated with 5 μmol/L MTI-31 alone or in combination with 10 μmol/L CQ or 0.01 μmol/L PS-341 for 24 h, then immunoblotted (left panels) and quantified (right panels). *P < 0.05; **P < 0.01, ***P < 0.001. (E) The amino acid sequence of TF was download from https://www.ncbi.nlm.nih.gov/to set up the model of TF structure using Pymol software. The KFERQ-motif is marked in yellow and its sequence is marked in red. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32923403), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Coagulation Factor III/Tissue Factor by Western Blot

Mechanistic target of rapamycin (mTOR) promotes TF overexpression in EGFR-mut tumors. (A) mTOR inhibitor WYE-132 and AZD8055 decreased TF expression in four indicated cell lines. The indicated cells were treated for 24 h in full growth medium followed by immunoblotting. (B) The indicated cells were serum-starved then stimulated with 50 ng/mL EGF without or with inhibitor for 24 h. (C) Serum-starved U87MG cells were stimulated with 10% serum without or with WYE-132 for 12 h and immunoblotted. (D,E) The indicated cell lines were infected with pGIPZ-shRNA encoding lentivirus, incubated for 6–7 days in full-growth medium and immunoblotted. (F) Cells as in D and E were serum-starved, stimulated with EGF and immunoblotted. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32923403), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Coagulation Factor III/Tissue Factor by Western Blot

Mechanistic target of rapamycin (mTOR) inhibitor differentially targets TF-mRNA transcription or TF protein degradation in a cellular context-dependent manner. (A,B) U87MG and HCC827 (A), H1975 and PC9 (B) cells were treated as indicated, subjected to qPCR analysis of TF mRNA as described in section “Materials and Methods.” The relative mRNA levels are shown. (C,D) H1975 (C) and PC9 (D) cells were treated with 5 μmol/L MTI-31 alone or in combination with 10 μmol/L CQ or 0.01 μmol/L PS-341 for 24 h, then immunoblotted (left panels) and quantified (right panels). *P < 0.05; **P < 0.01, ***P < 0.001. (E) The amino acid sequence of TF was download from https://www.ncbi.nlm.nih.gov/to set up the model of TF structure using Pymol software. The KFERQ-motif is marked in yellow and its sequence is marked in red. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32923403), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Coagulation Factor III/Tissue Factor by Western Blot

Mechanistic target of rapamycin (mTOR) promotes TF overexpression in EGFR-mut tumors. (A) mTOR inhibitor WYE-132 and AZD8055 decreased TF expression in four indicated cell lines. The indicated cells were treated for 24 h in full growth medium followed by immunoblotting. (B) The indicated cells were serum-starved then stimulated with 50 ng/mL EGF without or with inhibitor for 24 h. (C) Serum-starved U87MG cells were stimulated with 10% serum without or with WYE-132 for 12 h and immunoblotted. (D,E) The indicated cell lines were infected with pGIPZ-shRNA encoding lentivirus, incubated for 6–7 days in full-growth medium and immunoblotted. (F) Cells as in D and E were serum-starved, stimulated with EGF and immunoblotted. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32923403), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Coagulation Factor III/Tissue Factor by Western Blot

Mechanistic target of rapamycin (mTOR) promotes TF overexpression in EGFR-mut tumors. (A) mTOR inhibitor WYE-132 and AZD8055 decreased TF expression in four indicated cell lines. The indicated cells were treated for 24 h in full growth medium followed by immunoblotting. (B) The indicated cells were serum-starved then stimulated with 50 ng/mL EGF without or with inhibitor for 24 h. (C) Serum-starved U87MG cells were stimulated with 10% serum without or with WYE-132 for 12 h and immunoblotted. (D,E) The indicated cell lines were infected with pGIPZ-shRNA encoding lentivirus, incubated for 6–7 days in full-growth medium and immunoblotted. (F) Cells as in D and E were serum-starved, stimulated with EGF and immunoblotted. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32923403), licensed under a CC-BY license. Not internally tested by R&D Systems.