Detects human cIAP‑1/HIAP-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) cIAP-2 (aa 2‑604) and no cross-reactivity with rhBIRC6 (aa 4582‑4735), rhcIAP‑2 (aa 94‑178), rhXIAP (aa 1‑497), rhXIAP (BIR2 domain; aa 124‑242), or rhXIAP (BIR3 domain; aa 252‑356) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human cIAP-1/HIAP-2 Antibody

Detection of Human cIAP-1/HIAP-2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP-1/HIAP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human cIAP‑1/HIAP‑2 by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human cIAP-1/HIAP-2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for cIAP-1/HIAP-2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Applications for Human cIAP-1/HIAP-2 Antibody

Application

Recommended Usage

Knockout Validated

cIAP‑1/HIAP‑2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in cIAP‑1/HIAP‑2 knockout HeLa cell line.

Simple Western

5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB818 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cIAP-1/HIAP-2

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

References

Roy, N. et al. (1997) EMBO J. 23:6914.
Deveraux, Q. et al. (1997) Nature 388:300.
Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
Herrera, B. et al. (2002) FEBS Letters 520:93.

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Alternate Names

BIRC2, cIAP1, HIAP-2, MIHB

Entrez Gene IDs

329 (Human); 11797 (Mouse)

Gene Symbol

BIRC2

UniProt

Q13490

Additional cIAP-1/HIAP-2 Products

Product Documents for Human cIAP-1/HIAP-2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human cIAP-1/HIAP-2 Antibody

For research use only

Citations
Reviews

Human cIAP-1/HIAP-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB818

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications