Human CD20 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB42252
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry, Simple Western, COMET
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2709D
Product Specifications
Immunogen
Human CD20 Peptide. The epitope recognized by rituximab
Binder M, et al. (2006) 108:1975-8. doi: 10.1182/blood-2006-04-014639. Epub 2006 May 16. PMID: 16705086
Binder M, et al. (2006) 108:1975-8. doi: 10.1182/blood-2006-04-014639. Epub 2006 May 16. PMID: 16705086
Specificity
Detects human CD20 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CD20 Antibody
Detection of CD20 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™
CD20 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD20 Monoclonal Antibody (MAB42252) at 3ug/mL dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of B-cells. Protocol available in COMET™ Panel Builder.
Detection of Human CD20 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD20 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of CD20 in Human PBMCs by Flow Cytometry.
Human PBMCs were stained with (A) Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) or (B) Rabbit IgG Isotype Control Antibody (MAB1050), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human CD20 Antibody
Application
Recommended Usage
COMET
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC
Sample: Human PBMC
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Multiplex Immunofluorescence
3 µg/mL
Sample: Immersion fixed paraffin embedded sections of human tonsil
Sample: Immersion fixed paraffin embedded sections of human tonsil
Simple Western
25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line
Sample: Raji human Burkitt's lymphoma cell line
Western Blot
1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
Additional CD20 Products
Product Documents for Human CD20 Antibody
Product Specific Notices for Human CD20 Antibody
For research use only
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