Human C-Peptide Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB14171
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 790904
Product Specifications
Immunogen
Human C-Peptide synthetic peptide
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
Accession # P01308
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
Accession # P01308
Specificity
Detects human C-Peptide in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human C-Peptide Antibody
C-Peptide in BG01V Human Embryonic Stem Cells.
C-Peptide was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic beta cells using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB14171) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
C-Peptide in Human Pancreas.
C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB14171) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunofluorescent Staining of iPSC-derived Beta Islets.
iPSC-derived beta islets were fixed, frozen, and sectioned. Sections were stained with a Mouse Anti-Human C-peptide Monoclonal Antibody (Catalog # MAB14171), and a Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358), followed by secondary antibody staining with the NorthernLights NL493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009; green) and NorthernLights NL557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013; red). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid.Applications for Human C-Peptide Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into pancreatic beta cells
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into pancreatic beta cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Reviewed Applications
Read 1 review rated 5 using MAB14171 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Peptide
Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-Peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-Peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human C-Peptide shares 61% and 68% aa sequence identity with mouse and rat C-Peptide, respectively.
Long Name
Proinsulin C-Peptide
Alternate Names
C-P, Insulin C-Peptide, Proinsulin C-Peptide
Gene Symbol
INS
UniProt
Additional C-Peptide Products
Product Documents for Human C-Peptide Antibody
Product Specific Notices for Human C-Peptide Antibody
For research use only
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