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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant human CK1 epsilon
Met317-Lys416
Accession # P49674

Specificity

Detects endogenous human, mouse and rat CK1 epsilon in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha, CK1 delta, or CK1 gamma.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Casein Kinase 1 epsilon Antibody [CoraFluor™ 1]

Casein Kinase 1 epsilon Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Casein Kinase 1 epsilon Antibody [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Casein Kinase 1 epsilon

CKI epsilon is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI epsilon phosphorylates a large number of proteins and is involved in pathways involving Wnt and AKT. CKI epsilon has been found to bind and phosphorylate the circadian protein, Period, and the Wnt signaling protein, Dsh. In the Wnt pathway, CKI epsilon activity is required for the full activation of Dsh and downstream signaling effects.

Alternate Names

CSNK1E

Gene Symbol

CSNK1E

Additional Casein Kinase 1 epsilon Products

Product Documents for Casein Kinase 1 epsilon Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Casein Kinase 1 epsilon Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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