5-HT1B Antibody - BSA Free

Western Blot: 5-HT1B Antibody [NB100-56350]

Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.

Western Blot: 5-HT1B Antibody - BSA Free [NB100-56350] -

Exogenous serotonin did not block eosinophil transendothelial migration, consistent with intracellular HTR expression in endothelial cells. (A) Optical slices through the center of immunolabeled endothelial cell monolayers. Cells were fixed and immunolabeled with rabbit anti-mouse HTR1A, rabbit anti-mouse HTR1B or isotype control antibodies. Then the cells were washed and immunolabeled with FITC-conjugated goat anti-rabbit IgG. Then the cells were washed and immunolabeled with Alexa 647-conjugated rat anti-mouse VCAM-1 to mark the cell surface. The cells were cover-slipped with DAPI Prolong Gold to label the nuclei and coverslip the slides. The dotted white lines indicate location of cell membrane with VCAM-1 surface expression. (B) Biotinylation of endothelial surface proteins and western blot for HTR1A and HTR1B. (HTR1A, 46kD; HTR1B, 43kD; VCAM-1, 110kD; betaactin, 42kD). The vertical line in the anti-VCAM-1 blot indicates removal of an empty lane where no sample was loaded on the gel. (C) Exogenous serotonin treatment overnight and during the transendothelial migration assay did not block leukocyte transendothelial migration. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38845678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: 5-HT1B Antibody - BSA Free [NB100-56350] -

Endothelial cell HTR1A and HTR1B mediate 5HTP inhibition of eosinophil transendothelial migration. (A,B) HTR1A siRNA or HTR1B siRNA in endothelial cells knocked down HTR1A expression in the endothelial cells by western blot (HTR1A, 46kD; HTR1B, 43kD; betaactin, 42kD) as compared to non-target control siRNA. Shown are representative blots of 2 experiments. (C,D) siRNA of HTR1A or HTR1B in endothelial cells blocked the 5HTP (125 uM) inhibition of eosinophil transendothelial migration. N = 3 for representative experiment of 2 experiments. *p < 0.05 compared to control siRNA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38845678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.