Webinar: Going Beyond the Limits of Traditional Westerns: Detecting Low-Abundance Proteins in Your Model System

Detecting low-abundance proteins in physiologically relevant endogenous models is challenging. While traditional western blot is a foundational technique, it struggles with transfer losses, semi-quantitative readouts, long hands-on workflows, and lane-to-lane variability. Signals for low abundance-proteins frequently overlap with background, and variability from manual western blotting steps can make subtle expression changes difficult to interpret. These challenges are compounded by limited availability of precious samples to repeat experiments.

In this webinar, we'll highlight practical ways to push sensitivity and reproducibility for endogenous protein detection and tackle key biological challenges, such as detecting low-abundance proteins where traditional western blotting may fail. We’ll show how the enhanced sensitivity of Simple Western™ Technology paired with best-in-class antibodies from Cell Signaling Technology (CST) which are rigorously validated for application-specific specificity and reproducibility, overcomes these methodological barriers to deliver quantitative results from minimal sample in ~3 hours. This approach demonstrates how Simple Western enables new applications for antibodies previously designated as 'transfected-only'—antibodies that reliably detect overexpressed recombinant protein but lack the sensitivity for consistent detection of endogenous targets by traditional Western blot.

Francisco Ramirez from Bio-Techne will introduce the Simple Western Platform and assay design considerations. Dr. Chris LaBreck from CST will share data demonstrating how highly validated antibodies, combined with a highly sensitive workflow, enable confident, quantitative detection of low-abundance endogenous proteins in native systems.