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Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
| Validated Target | Host Species | Vendor / Cat # | Sample | Concentration |
|---|---|---|---|---|
| α-actinin | Goat |
R&D Systems AF8279 |
KPC cells | 100 µg/mL |
| α-actinin4 | Mouse |
Santa Cruz Biotechnology sc-390205 |
HeLa cells | 100 µg/mL |
| α-synuclein | Rabbit |
Cell Signaling 4179 |
SHY5Y cells | 1:10 |
| β-tubulin | Mouse |
Genetex GTX11312 |
Primary Her2+ breast tumor cells | 1:10 |
| β-tubulin* | Rabbit |
Abcam ab6046 |
Murine pancreatic cancer cells | 1:40 |
| β-tubulin* | Mouse |
Genscript A-01717-40 |
Human pluripotent stem cells | 1:40 |
| βIII-tubulin (neuronal) | Mouse |
Sigma Aldrich T8578 |
Human iPSCs | 100 µg/mL |
| Actin | Mouse |
Developmental Studies Hybridoma Bank 224-236-1 |
Dictyostelium discoideum | 100 µg/mL |
| Akt (pan) | Mouse |
Cell Signaling 2920 |
C2C12 cells | 1:10 |
| Akt (pan) | Rabbit |
Cell Signaling 4685 |
C2C12 cells | 1:10 |
| Akt (pan)* | Rabbit |
Cell Signaling 4691 |
C2C12 cells | 1:10 |
| Albumin | Mouse |
Abcam 10241 |
Hepatocyte co-culture | 100 µg/mL |
| Albumin | Rabbit |
Cell Signaling 4929 |
Hepatocyte co-culture | 1:10 |
| ALDH1A1 | Rabbit |
Cell Signaling 12035 |
(unpublished) | 1:10 |
| alpha-actinin | Rabbit |
Cell Signaling Technology 6487 |
BT474 | |
| AML1 | Rabbit |
Cell Signaling 4336 |
Jurkat cells | 1:10 |
| APRIL | Goat |
R&D Systems AF884 |
MOLT-4 cells | 1:5 |
| ASC | Rabbit |
Adipogen AG-25B-0006-C100 |
GM-CSF BMDCs | 50 µg/mL |
| ATG5 | Rabbit |
Cell Signaling Technology CST129945 |
Macrophages | 1:10 |
| Atg7 | Mouse |
R&D Systems MAB6608 |
HeLa cells | 100 µg/mL |
| ATG7 | Mouse |
R&D Systems MAB6608 |
Macrophages | 1:10 |
| Atg7* | Rabbit |
Abcam ab133528 |
HeLa cells | 100 µg/mL |
| ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
| ATP1A3 | Mouse |
Abcam ab2826 |
Live primary human cortical neurons | 100 µg/mL |
| ATP1A3/Sodium Potassium ATPase Alpha 3 | Mouse Monoclonal |
Novus Biologicals NB300-540 |
Live primary human cortical neurons | 100 µg/mL |
| Axl | Rabbit |
Cell Signaling 8661 |
HeLa cells | 1:10 |
| BAFF | Goat |
R&D Systems AF124 |
MOLT-4 cells | 1:10 |
| Bcl-xL | Rabbit |
Cell Signaling 2764 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling 5650 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling 14895 |
HeLa cells | 1:10 |
| beta-tubulin | Mouse |
Novus Biologicals NBP2-50061 |
Jurkat cells | 50 µg/mL |
| beta-tubulin | Mouse |
Novus Biologicals NB120-7792 |
Jurkat cells | 50 µg/mL |
| betaIII-tubulin | Mouse |
R&D Systems MAB1195 |
neural differentiated iPSCs | 100 µg/mL |
| Blasticidin | Rabbit |
Abcam ab38307 |
Transfected HEK293T cells | 100 µg/mL |
| Blimp-1 | Rabbit |
Cell Signaling 9115 |
U266 cells | 1:10 |
| BRM | Rabbit |
Cell Signaling 11966 |
293T cells | 1:10 |
| BTK | Rabbit |
Cell Signaling 8547 |
Primary sorted human spleen lymphocytes | 1:10 |
| BTK | Mouse |
Cell Signaling 56044 |
Primary sorted human spleen lymphocytes | 1:10 |
| c-Jun | Rabbit |
Cell Signaling 9165 |
KPC cells | 1:10 |
| c-Myc | Rabbit |
Cell Signaling 5605 |
Human pluripotent stem cells | 1:10 |
| Calnexin | Rabbit |
Novus Biologicals NBP1-97476 |
U373 MG cells | 1:10 |
| Calnexin | Rabbit |
Enzo Life Sciences ADI-SPA-865 |
U373 MG cells | 1:10 |
| Cas9 | Mouse |
Abcam ab191468 |
Mouse lymphocytes | 1:10 |
| Caspase 8 | Mouse |
Enzo ADI-AAM-118-E |
Treated Jurkat cells | 1:10 |
| CCL3/MIP1 | Goat |
R&D Systems AF-270-NA |
Differentiated THP-1 treated with LPS and Transport Inhibitors | 20 µg/mL |
| CCR7 | Rabbit |
Novus Biologicals NBP2-58753 |
Jurkat cells | 100 µg/mL |
| CD-80 (B7-1) | Goat |
R&D Systems AF140 |
Dendritic cells | 100 µg/mL |
| CD11b | Rabbit |
Novus Biologicals NB110-89474 |
(unpublished) | 1:10 |
| CD19 | Rabbit |
Cell Signaling 3574 |
Ramos cells | 1:10 |
| CD209 (DC-SIGN) | Sheep |
R&D Systems AF161 |
Dendritic cells | 100 µg/mL |
