Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
Filter Antibodies: (415)
| Validated Target | Host Species | Vendor / Cat # | Sample | Concentration |
|---|---|---|---|---|
| α-actinin | Goat |
R&D Systems AF8279 |
KPC cells | 100 µg/mL |
| α-actinin4 | Mouse |
Santa Cruz Biotechnology sc-390205 |
HeLa cells | 100 µg/mL |
| α-synuclein | Rabbit |
Cell Signaling Technology 4179 |
SHY5Y cells | 1:10 |
| α-Tubulin | Mouse |
Santa Cruz Biotechnology sc-8035 |
Not stated | 1:3000 |
| β-tubulin | Mouse |
Genetex GTX11312 |
Primary Her2+ breast tumor cells | 1:10 |
| β-tubulin* | Mouse |
Genscript A-01717-40 |
Human pluripotent stem cells | 1:40 |
| β-tubulin* | Rabbit |
Abcam ab6046 |
Murine pancreatic cancer cells | 1:40 |
| βIII-tubulin (neuronal) | Mouse |
Sigma Aldrich T8578 |
Human iPSCs | 100 µg/mL |
| ABCG2 | Mouse |
Santa Cruz Biotechnology sc-58222 |
Placenta | 1:500 |
| Actin | Mouse |
Developmental Studies Hybridoma Bank 224-236-1 |
Dictyostelium discoideum | 100 µg/mL |
| Akt (pan) | Mouse |
Cell Signaling Technology 2920 |
C2C12 cells | 1:10 |
| Akt (pan) | Rabbit |
Cell Signaling Technology 4685 |
C2C12 cells | 1:10 |
| Akt (pan) (C67E7) | Rabbit |
Cell Signaling Technology 4691 |
CRC tumor samples and human cell lines | 1:10 |
| Akt (pan)* | Rabbit |
Cell Signaling Technology 4691 |
C2C12 cells | 1:10 |
| Albumin | Mouse |
Abcam 10241 |
Hepatocyte co-culture | 100 µg/mL |
| Albumin | Rabbit |
Cell Signaling Technology 4929 |
Hepatocyte co-culture | 1:10 |
| ALDH1A1 | Rabbit |
Cell Signaling Technology 12035 |
(unpublished) | 1:10 |
| alpha-actinin | Rabbit |
Cell Signaling Technology 6487 |
BT474 | |
| AML1 | Rabbit |
Cell Signaling Technology 4336 |
Jurkat cells | 1:10 |
| Anti-Mouse Alexa Fluor 555 | Goat |
ThermoFisher A-21422 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-Mouse Alexa Fluor 647 | Goat |
ThermoFisher A-21235 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-Mouse IgG (H+L) Alexa Fluor 488 | Donkey |
Invitrogen Antibodies A-21202 |
Mammary glands | Not stated |
| Anti-Mouse IgG (H+L) Alexa Fluor 555 | Donkey |
Invitrogen Antibodies A-31570 |
Not stated | Not stated |
| Anti-Mouse IgG (H+L) Alexa Fluor 647 | Donkey |
Invitrogen Antibodies A-31571 |
Stem cell | 1:20 |
| Anti-Mouse IgG Fab2 Alexa Fluor 555 | Goat |
Cell Signaling Technology 4409 |
(unpublished) | 1:20 |
| Anti-Rabbit Alexa Fluor 555 | Goat |
ThermoFisher A-21428 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-Rabbit Alexa Fluor 647 | Chicken |
ThermoFisher A-21443 |
CRC tumor samples and human cell lines | 1:20 |
| Anti-Rabbit IgG (H+L) Alexa Fluor 532 | Goat |
Invitrogen Antibodies A-11009 |
NIH 3T3 | 1:20 |
| Anti-Rabbit IgG (H+L) Alexa Fluor 647 | Donkey |
Invitrogen Antibodies A-31573 |
Mammary glands | Not stated |
| Anti-Rabbit IgG (H+L) Alexa Fluor 647 | Donkey |
Invitrogen Antibodies A-31573 |
Not stated | Not stated |
| Anti-Rat IgG (H+L) Alexa Fluor 647 | Goat |
Invitrogen Antibodies A-21247 |
NIH 3T3 | 1:20 |
| APRIL | Goat |
R&D Systems AF884 |
MOLT-4 cells | 1:05 |
| ASC | Rabbit |
Adipogen AG-25B-0006-C100 |
GM-CSF BMDCs | 50 µg/mL |
| ATG5 | Rabbit |
Cell Signaling Technology 129945 |
Macrophages | 1:10 |
| Atg7 | Mouse |
R&D Systems MAB6608 |
HeLa cells | 100 µg/mL |
| Atg7* | Rabbit |
Abcam ab133528 |
HeLa cells | 100 µg/mL |
| ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
| ATP1A3 | Mouse |
Abcam ab2826 |
Live primary human cortical neurons | 100 µg/mL |
| ATP1A3/Sodium Potassium ATPase Alpha 3 | Mouse |
Novus Biologicals NB300-540 |
Live primary human cortical neurons | 100 µg/mL |
| Axl | Rabbit |
Cell Signaling Technology 8661 |
HeLa cells | 1:10 |
| BAFF | Goat |
R&D Systems AF124 |
MOLT-4 cells | 1:10 |
| Bcl-xL | Rabbit |
Cell Signaling Technology 2764 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling Technology 14895 |
HeLa cells | 1:10 |
| BCL6 | Rabbit |
Cell Signaling Technology 5650 |
HeLa cells | 1:10 |
| beta-tubulin | Mouse |
Novus Biologicals NB120-7792 |
Jurkat cells | 50 µg/mL |
| beta-tubulin | Mouse |
Novus Biologicals NBP2-50061 |
Jurkat cells | 50 µg/mL |
| betaIII-tubulin | Mouse |
R&D Systems MAB1195 |
neural differentiated iPSCs | 100 µg/mL |
| Blasticidin | Rabbit |
Abcam ab38307 |
Transfected HEK293T cells | 100 µg/mL |
| Blimp-1 | Rabbit |
Cell Signaling Technology 9115 |
U266 cells | 1:10 |
| BRM | Rabbit |
Cell Signaling Technology 11966 |
293T cells | 1:10 |