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Milo Antibody Database
Find antibodies validated for Single-Cell Westerns on Milo
Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
Filter Antibodies: (395)
| Validated Target | Host Species | Vendor / Cat # | Sample | Concentration |
|---|---|---|---|---|
| α-actinin | Goat |
R&D Systems AF8279 |
KPC cells | 100 µg/mL |
| β-tubulin* | Rabbit |
Novus Biologicals NB600-936 |
Mouse dendrites | 1:40 |
| APRIL | Goat |
R&D Systems AF884 |
MOLT-4 cells | 1:5 |
| Atg7 | Mouse |
R&D Systems MAB6608 |
HeLa cells | 100 µg/mL |
| ATG7 | Mouse |
R&D Systems MAB6608 |
Macrophages | 1:10 |
| ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
| ATP1A3/Sodium Potassium ATPase Alpha 3 | Mouse Monoclonal |
Novus Biologicals NB300-540 |
Live primary human cortical neurons | 100 µg/mL |
| BAFF | Goat |
R&D Systems AF124 |
MOLT-4 cells | 1:10 |
| beta-tubulin | Mouse |
Novus Biologicals NBP2-50061 |
Jurkat cells | 50 µg/mL |
| beta-tubulin | Mouse |
Novus Biologicals NB120-7792 |
Jurkat cells | 50 µg/mL |
| betaIII-tubulin | Mouse |
R&D Systems MAB1195 |
neural differentiated iPSCs | 100 µg/mL |
| Calnexin | Rabbit |
Novus Biologicals NBP1-97476 |
U373 MG cells | 1:10 |
| CCL3/MIP1 | Goat |
R&D Systems AF-270-NA |
Differentiated THP-1 treated with LPS and Transport Inhibitors | 20 µg/mL |
| CCR7 | Rabbit |
Novus Biologicals NBP2-58753 |
Jurkat cells | 100 µg/mL |
| CD-80 (B7-1) | Goat |
R&D Systems AF140 |
Dendritic cells | 100 µg/mL |
| CD11b | Rabbit |
Novus Biologicals NB110-89474 |
(unpublished) | 1:10 |
| CD209 (DC-SIGN) | Sheep |
R&D Systems AF161 |
Dendritic cells | 100 µg/mL |
| CD25 | Goat |
R&D Systems AF-223-NA |
Tregs | 100 µg/mL |
| CD29 | Goat |
R&D Systems AF2405 |
KPC cells | 100 µg/mL |
| CD4 | Goat |
R&D Systems AF-379-NA |
PBMCs | 1:10 |
| CD56 (NCAM-1) | Goat |
R&D Systems AF2408 |
Differentiated NK cells | 100 µg/mL |
| CD68 | Rat |
Novus Biologicals NBP2-33337 |
RAW264.7 cells | 100 µg/mL |
| CD68/SR-D1 | Rat |
Novus Biologicals NBP2-80656 |
RAW264.7 cells | 100 µg/mL |
| CD81 | Mouse |
Novus Biologicals NBP2-53318 |
Gli36 cells | 100 µg/mL |
| CD81* | Mouse |
Novus Biologicals NBP1-44861 |
Gli36 cells | 100 µg/mL |
| CD83 | Goat |
R&D Systems AF2044 |
Dendritic cells | 100 µg/mL |
| CD86 (B7-2) | Goat |
R&D Systems AF-141-NA |
Dendritic cells | 100 µg/mL |
| CRBN | Rabbit |
Novus Biologicals NBP1-91810 |
HeLa cells | 100 µg/mL |
| CRMP5 | Rat |
Novus Biologicals NB100-74395 |
1:10 | |
| Cyclin D1 | Rabbit |
R&D Systems AF4196 |
MCF-7 cells | 100 µg/mL |
| Cyclophilin B | Goat |
R&D Systems AF5410 |
HEK293T cells | 100 µg/mL |
| Cytochrome b reductase 1 | Rabbit |
Novus Biologicals NBP2-16075 |
Red blood cells | 100 µg/mL |
| DLL4 | Goat |
R&D Systems AF1389 |
KPC cells | 100 µg/mL |
| DNMT3A | Goat |
R&D Systems AF6315 |
SH-SY5Y | 20 µg/mL |
| E-cadherin | Goat |
R&D Systems AF748 |
KPC cells | 100 µg/mL |
| EGFR* | Goat |
R&D Systems AF231 |
BEAS-2B cells | 100 µg/mL |
| EpCAM* | Goat |
R&D Systems AF960 |
(unpublished) | 100 µg/mL |
| EphB4 | Goat |
R&D Systems AF446 |
Neural stem cells | 1:20 |
| FAK | Sheep |
R&D Systems AF4467 |
KPC cells | 100 µg/mL |
| FLAG Tag | Rabbit |
R&D Systems MAB8529 |
3T3 Flag-ATF6 cells | 100 µg/mL |
| FOXP3 | Goat |
R&D Systems BAF3240 |
Mouse T-regs | |
| FOXP3 | Rabbit |
Novus Biologicals NB100-39002 |
FoxP3-transfected HEK293T | 100 µg/mL |
| GAPDH | Mouse |
Novus Biologicals NB300-221 |
freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein | 1:40 |
| GAPDH | Goat |
R&D Systems AF5718 |
HT-29 cells | 100 µg/mL |
| GAPDH | Goat |
Novus Biologicals NB300-320 |
Jurkat cells | |
| GATA-3 | Mouse |
R&D Systems MAB6330 |
MCF-7 cells | 100 µg/mL |
| GATA-3 | Mouse |
R&D Systems MAB26051 |
MCF-7 cells | 100 µg/mL |
| GATA-3* | Goat |
R&D Systems AF2605 |
MCF-7 cells | 100 µg/mL |
| GDNF | Goat |
R&D Systems AF-212-NA |
U-87 MG | 20 µg/mL |
| GFAP | Sheep |
R&D Systems AF2594 |
Human iPSCs | 1:20 |
