beta Tubulin Antibody (TU-06) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7792
![Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-TU-06-Western-Blot-NB120-7792-img0002.jpg "Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, A. thaliana, Chicken, Eukaryote, Fish, Plant
Cited:
Rat, Plant
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TU-06
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Beta-subunits of porcine brain tubulin.
Reactivity Notes
Expected species cross-reactivity based on sequence homology: Carp, Bovine, Hamster, S.Cerevisiae, S.Pombe, and Protozoa. Plant reactivity reported in scientific literature (PMID: 26594217).
Localization
Cytoplasmic
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for beta Tubulin Antibody (TU-06) - BSA Free
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792] - Analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 ug/ml, 1 ug/ml, 2 ug/ml, 4 ug/ml, 5 ug/ml).![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]](https://resources.bio-techne.com/images/products/beta-Tubulin-Antibody-TU-06-Immunocytochemistry-Immunofluorescence-NB120-7792-img0003.jpg "Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]")
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy(R)5). Nucleus is stained with DAPI (blue).Applications for beta Tubulin Antibody (TU-06) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunocytochemistry: fixed and permeabilized cells; positive control: 3T3 mouse embryonal fibroblast cell line. Immunohistochemistry (paraffin sections) positive tissue: heart.
Reviewed Applications
Read 1 review rated 4 using NB120-7792 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
Tris buffered saline (TBS), pH 8.0
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB
UniProt
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody (TU-06) - BSA Free
Product Specific Notices for beta Tubulin Antibody (TU-06) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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