What are Simple Plex Ultra-Sensitive Assays?
From discovery to clinical research, Simple Plex Ultra-Sensitive Assays simplify biomarker detection while delivering robust, reproducible measurement of low abundant proteins. This advancement enables reliable detection and precise quantification of endogenous biomarkers in plasma and serum samples —without added complexity.
- Ultra sensitivity – Measure biomarkers at endogenous levels with confidence, enabling earlier insights and more precise decision-making.
- Built-in standard curves – Save time and reduce errors with automated standard curves, ensuring consistent, reproducible results across every run.
- Validated for key sample types – Get reliable results from the most commonly used sample types including CSF, serum, and plasma so you can focus on discovery without worrying about compatibility or validation.
- Fast results in under 3 hours – Get actionable data quickly, accelerating your research timelines and improving productivity without sacrificing accuracy.
High detectability of neurodegeneration biomarkers in healthy plasma, at ultra-low concentrations. Neurodegenerative biomarkers were quantified in plasma samples from healthy donors (age 55+), using the Simple Plex Ultra-Sensitive Assay. The results indicate full detectability and near complete quantifiability of all samples across all assays.
Simple Plex Ultra-Sensitive Assay Technology
How it Works
- Sample is routed through a microfluidic circuitry, with synchronized release of assay reagents.
- Sandwich immunoassay occurs within Glass Nano Reactors (GNRs).
Achieving ultra-sensitive detection
- Assay parameters and microfluidic protocols were systematically optimized to achieve femtogram-level sensitivity.
- The platform’s high precision minimizes variability at the low end of the standard curve, enabling more reliable quantification of low abundance analytes.
Available Simple Plex Assays Comparison
| Discovery Simple Plex Assays Early Research Testing | Simple Plex Assays Highly Validated Kits | Ultra-Sensitive Simple Plex Assays Highly validated kits for ultra-sensitive detection | |
|---|---|---|---|
| Recommended When | Less stringent assay specifications for discovery and early research | Precise and accurate results with tight performance specifications for regulated environments | Sensitive and accurate results for detection of low-abundant proteins at healthy endogenous levels |
| Sensitivity | sub pg/ml | sub pg/ml | fg/ml |
| Assay Run Time | 75 min | 75 min | 155 min |
| Required Sample Volume | ≤25 µL | ≤25 µL | ≤40 µL |
| Species (currently available) | Human, Mouse, Rat | Human, Mouse, Rat, Non-Human Primate | Human |
| Multiplex | Up to 8 | Up to 8 | Up to 4 (limited by available analytes) |
| Assay Performance | Intra-assay: ≤12% Inter-assay: ≤12% | Extensively tested throughout manufacture and validated to ensure long-term consistency Intra-assay: ≤10% | Extensively tested throughout manufacture and validated to ensure long-term consistency Intra-assay: ≤10% |
| Precision | Verified across 2 manufactured lots | Validated across 3+ manufactured lots | Validated across 3+ manufactured lots |
| Linearity | Range 75-125% Verified across 2 manufactured lots | Range 80-120% Validated across 3+ manufactured lots | Range 80-120% Validated across 3+ manufactured lots |
| Recovery | Range 75-125% Verified across 2 manufactured lots | Range 80-120% Validated across 3+ manufactured lots | Range 80-120% Validated across 3+ manufactured lots |
| Assay Validation | Verified with recombinant proteins | Verified recombinant proteins and natural samples | Verified recombinant proteins and natural samples |
Resources
Sensitive Neuro Biomarker Detection
Sensitive Neuro Biomarker Detection
Simple Plex Assays have been extensively validated in numerous studies, demonstrating exceptional sensitivity and precision for detecting low abundance proteins. Fluorescent detection improves sensitivity by 10X and boosts dynamic range up to 5 logs while the fully automated assay workflow delivers results in less than 3 hrs.
Rethink Your Biomarker Detection With Ella
Rethink Your Biomarker Detection With Ella
Standard ELISA techniques for detecting protein biomarkers often come up short in terms of sensitivity and reproducibility. Ella automates your assay workflow, delivering accurate and consistent data — without the manual set up common with traditional ELISAs. With Ella’s compact footprint and hands-free workflow, you get accurate data with no manual steps.
Immunoassays for Neuroinflammation
Immunoassays for Neuroinflammation
The field of research into neurodegeneration and various neurological disorders is growing rapidly. As the range of treatment options broaden and attention turns to early detection, there arises a demand for improved, non-invasive methods to track neuro biomarkers and neuroinflammatory cytokines. In this guide, you’ll find the optimal assays for your neuroinflammation research.
Related Products
| Simple Plex Assays | Catalog Number | Simple Plex Assays | Catalog Number |
|---|---|---|---|
| Simple Plex Ultra Phospho-Tau (T217) | SPCKB-PS-015536 | Simple Plex Ultra NFL | SPCKB-PS-015538 |
| Simple Plex Amyloid Beta (aa1-42) | SPCKB-PS-015537 | Simple Plex Ultra GFAP | SPCKB-PS-015539 |
| Ella System | 600-100 |
