Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015537
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Amyloid beta (aa1-42) assay.
Inter-Assay Precision (Precision between assays) Replicates of each Amyloid beta (aa1-42) control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Amyloid beta (aa1-42).
Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 95 | 93 |
| Mean (pg/mL) | 12.1 | 534 | 11.5 | 557 |
| Standard Deviation | 0.696 | 23.9 | 1.26 | 59.9 |
| CV% | 5.8 | 4.5 | 10.9 | 10.8 |
Recovery for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay
Recovery was not necessary as human Amyloid beta (aa1-42) shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of Amyloid beta (aa1-42) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay
Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay. Assay range is 0.92-1409 pg/mL.
Simple Plex Ultra-Sensitive Human Amyloid beta (aa1-42) Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Preparation and Storage
Shipping
Stability & Storage
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
Alternate Names
Gene Symbol
Additional Amyloid beta Products
Product Documents for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay
Product Specific Notices for Simple Plex Ultra-Sensitive Human Amyloid Beta aa1-42 Assay
Software Requirements: The Simple Plex Ultra-Sensitive Assays require Runner/Explorer 5.0 and Windows 11.
For research use only