Simple Plex Ultra-Sensitive Human NF-L Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-015538
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Ultra-Sensitive Human NF-L Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one NF-L assay.
Inter-Assay Precision (Precision between assays) Replicates of each NF-L control were tested in multiple assays performed by at least three technicians using two lots of reagents for human NF-L.
Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 90 | 86 |
| Mean (pg/mL) | 66.2 | 3031 | 77.1 | 3280 |
| Standard Deviation | 1.21 | 78.3 | 8.44 | 205 |
| CV% | 1.8 | 2.6 | 10.9 | 6.3 |
Recovery for Simple Plex Ultra-Sensitive Human NF-L Assay
Recovery for the human NF-L assays was evaluated at three different spiked concentrations of NF-L protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=5) | 86 | 78-97 |
| Heparin Plasma (n=4) | 93 | 88-99 |
| Serum (n=4) | 83 | 67-101 |
Linearity
Samples containing and/or spiked with high concentrations of NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Ultra-Sensitive Human NF-L Assay
Simple Plex Ultra-Sensitive Human NF-L Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Ultra-Sensitive Human NF-L Assay. Assay range is 1.99-7600 pg/mL.
Simple Plex Ultra-Sensitive Human NF-L Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Preparation and Storage
Shipping
Stability & Storage
Background: NF-L
Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.
All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional NF-L Products
Product Documents for Simple Plex Ultra-Sensitive Human NF-L Assay
Product Specific Notices for Simple Plex Ultra-Sensitive Human NF-L Assay
Software Requirements: The Simple Plex Ultra-Sensitive Assays require Runner/Explorer 5.0 and Windows 11.
For research use only