Rat Cortical Stem Cells (3 x 10e6 cells/vial)
R&D Systems, part of Bio-Techne | Catalog # NSC001
Key Product Details
Species
Rat
Product Summary for Rat Cortical Stem Cells (3 x 10e6 cells/vial)
Kit Summary
Why use Pre-isolated Fully Characterized Rat Cortical Stem Cells?
Although there are a number of protocols available to isolate rat cortical stem cells, each isolation can yield cells that vary in yield, purity, and their ability to proliferate. See Details
R&D Systems offers Rat Cortical Stem Cells that are of high purity, frozen at a Passage 0, and are validated to be free of mycoplasma and microbial contamination. Rat primary cortical stem cells were isolated from the cortex of embryonic E14.5 Sprague-Dawley rats. The cells were cultured in a monolayer system in medium supplemented with N-2 Plus Media Supplement (Catalog # AR003) or N-2 MAX Media Supplement (Catalog # AR009) and Recombinant Human FGF basic (Catalog # 233-FB or 4114-TC). Cells were then harvested and cryopreserved. The cells are designated as passage 0 (P0) cells.
R&D Systems Rat Cortical Stem Cells:
- Have been fully characterized to reduce experimental variation.
- Have been shown to differentiate into neurons, astrocytes, and oligodendrocytes.
- Can be expanded as monolayers or neurospheres.
- Are free of mycoplasma and microbial contamination.
Components
1 vial of Rat Cortical Stem Cells containing 3 x 106 cells. See Details
Storage
Store in liquid nitrogen for up to 1 year.
Quality Control
- Cells from this lot have been thawed and tested for their ability to proliferate using either a monolayer system (for 3 passages) or a neurosphere system (for 4 passages). Stem and progenitor cells expanded from the end of passage 3 (monolayer system) or passage 4 (neurosphere system) have been examined for Nestin and SOX2 expression. They were also tested for their ability to differentiate into astrocytes, neurons, and oligodendrocytes.
- The cells tested negative for mycoplasma using the MycoProbe™ Mycoplasma Detection Kit (Catalog # CUL001B). The cells also tested negative for microbial contamination.
Precautions
This product contains trace amounts of DMSO and human transferrin. The transferrin was purified from plasma that was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV 1/2 and Hepatitis B surface antigen.
Scientific Data Images for Rat Cortical Stem Cells (3 x 10e6 cells/vial)
Verification of Neural Progenitor Cell Multipotency Following Expansion with N-2 MAX Media Supplement.
Rat Cortical Stem Cells were differentiated for 7 days in media supplemented with N-2 MAX Media Supplement (Catalog # AR009). Differentiated cells were stained with a Mouse Neuron-specific Anti-beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by the NorthernLights™ (NL)557-conjugated Secondary Antibody (Catalog # NL007; red) to detect neurons. The cells were stained with a Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by the NL557-conjugated Secondary Antibody (red; Catalog # NL010) to detect astrocytes; and cells were stained with a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326), followed by the NL557-conjugated Secondary Antibody (Catalog # NL019) to detect oligodendrocytes. The nuclei were counterstained with DAPI (blue).
Neural Progenitor Cells Expanded with N-2 Plus Media Supplement Express Nestin and SOX2.
Rat Cortical Stem Cells (Catalog # NSC001) were cultured for 7 days in media supplemented with 1X N-2 Plus Media Supplement (Catalog # AR003) and 20 ng/mL of Recombinant Human FGF basic (Catalog # 233-FB). The cells were stained with a PE-conjugated Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # IC1259P, red histogram), a PE-conjugated Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # IC2018P, green histogram), or a PE-conjugated Mouse IgG2A Isotype Control Antibody (Catalog # IC003P, open histogram). Under these conditions, cells were shown to express high levels of neural multipotency markers Nestin and SOX2.
Differentiation of Rat Cortical Stem Cells.
Neural progenitors were cultured for seven days in DMEM/F12 containing N-2 Plus Media Supplement (Catalog # AR003) without FGF Basic to induce differentiation. The differentiated cells were labeled with a Goat Anti-Rat Nestin Affinity-purified Polyclonal Antibody (Catalog # AF2736) followed by the NorthernLights™(NL)493-conjugated Donkey Anti-Goat IgG Secondary Antibody (Catalog # NL003; green) and a Mouse Neuron-specific Anti-beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by the NL-557-conjugated Donkey IgG Anti-Mouse Secondary Antibody (Catalog # NL007; red). Nuclei were counterstained with DAPI (blue).Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store in liquid nitrogen for up to 12 months from the date of receipt.
Background: Neural Stem Cells
Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.
To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
Additional Neural Stem Cells Products
Product Documents for Rat Cortical Stem Cells (3 x 10e6 cells/vial)
Product Specific Notices for Rat Cortical Stem Cells (3 x 10e6 cells/vial)
For research use only
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