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Human/Rat GFAP Antibody
Catalog # AF2594 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Summary for Human/Rat GFAP Antibody
Immunogen
E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136
Leu292-Met432
Accession # P14136
Specificity
Detects human and rat GFAP in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Rat GFAP Antibody
Detection of Human and Rat GFAP by Western Blot.
Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes.
beta-III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern-Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GFAP in Rat Astrocytes.
GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human/Rat GFAP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed rat astrocytes and immersion fixed 7 days differentiated rat cortical stem cells
Sample: Immersion fixed rat astrocytes and immersion fixed 7 days differentiated rat cortical stem cells
Simple Western
0.1-2 µg/mL
Sample: Human brain (cerebellum) tissue and rat brain tissue
Sample: Human brain (cerebellum) tissue and rat brain tissue
Western Blot
0.2 µg/mL
Sample: Rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue
Sample: Rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF2594 in the following applications:
Published Applications
Read 8 publications using AF2594 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFAP
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.
Product Documents for Human/Rat GFAP Antibody
Product Specific Notices for Human/Rat GFAP Antibody
For research use only
Citations for Human/Rat GFAP Antibody (8)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Rat GFAP Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCOliver et al. (2023-06-21), Dengue Virus Infection of Human Retinal Müller Glial Cells Viruses
PMID: 37515098 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Su et al. (2022-11-03), A single-cell transcriptome atlas of glial diversity in the human hippocampus across the postnatal lifespan Cell Stem Cell, 2022-129(11):1594-1610.e8.
PMID: 36332572 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCK Wang et al. (2021-12-16), Anti-M�llerian Hormone Regulation of Synaptic Transmission in the Hippocampus Requires MAPK Signaling and Kv4.2 Potassium Channel Activity Frontiers in Neuroscience, 2021-115(0):772251.
PMID: 34975379 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKM LaBarbera et al. (2021-04-06), Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery Journal of neuroscience methods, 2021-00(0):109180.
PMID: 33836174 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PC Liu et al. (2017-12-13), APP upregulation contributes to retinal ganglion cell degeneration via JNK3 Cell Death Differ., 2017-10(0).
PMID: 29238071 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKT Gurwitz et al. (2017-05-29), Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders Front Mol Neurosci, 2017-010(0):163.
PMID: 28611588 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot(2012-05-23), Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012-013(0):50.
PMID: 22621398 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCLeonoudakis D et al. (2004-08-01), TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity? Neuron Glia Biol., 2004-01(3):263-273.
PMID: 16520832
There are no citations that match your criteria.
Customer Reviews for Human/Rat GFAP Antibody (1)
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