Bio-Techne’s VisUCyte™ HRP Polymer Detection Kits are an advanced research solution for streamlined and precise tissue staining. Our kits, consisting of biotin-free detection reagents, overcome challenges of avidin-biotin detection chemistry by providing faster and more efficient, specific staining on tissue sections. With the high sensitivity of VisUCyte™ HRP Polymer, primary antibody concentrations can be reduced by 2-3 times compared to conventional methods.
Our HRP polymer-conjugated secondary antibodies are versatile, reacting with all IgG subclasses, suitable for both manual protocols and tissue autostainers. Secondary antibodies used for conjugation to HRP polymer undergo rigorous quality control, ensuring reproducibility. Due to their high sensitivity, VisUCyte™ kits are ideal for detecting low-abundant tissue proteins, providing the ability to complete IHC experiments in just 1-2 hours, while additionally resulting in significant cost savings.
Download the datasheet to read full technical specifications, staining procedure, and more.
Scientific Data Examples for Mouse VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit
Detection of Vimentin in Human Testis.
Tissue sections were first incubated with Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (MAB21052) and then subjected to staining development using Mouse IgG VisUCyte HRP-DAB Kit (Catalog # VCTS001B). Specific staining was localized to the cytoplasm. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.
Detection of CK 14 in Human Skin
Tissue sections were first incubated with Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (MAB3164) and then subjected to staining development using Mouse VisUCyte Detection kit (Catalog# VCTS001B). Staining is confined to keratinocytes and hair follicles. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.
Side-by-side comparison of staining using different detection chemistries on adjacent tissue sections of Human Lung Tumor.
Vimentin was detected in immersion fixed paraffin-embedded sections of human lung tumor using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 1 µg/ml overnight at 4 °C followed by incubation with the Mouse VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (Catalog # VCTS001B) and a generic Avidin-biotin kit. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. Strong specific tissue staining is seen using VisUCyte kit. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Side-by-side comparison of staining using different detection chemistries on adjacent tissue sections of Human Pancreas Tumor.
Vimentin was detected in immersion fixed paraffin-embedded sections of human pancreas tumor using Mouse Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB21052) at 1 µg/ml overnight at 4 °C followed by incubation with the Mouse VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (Catalog # VCTS001B) and a generic Avidin-Biotin kit. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. Strong specific tissue staining is seen using VisUCyte kit. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation & Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
