dTAG^V-1

Name: dTAG V -1
Brand: Tocris Bioscience
SKU: 6914

Tocris Bioscience a Bio-Techne Brand

Catalog # 6914

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Key Product Details

Description:	Potent and selective degrader of mutant FKBP12^F36V fusion proteins
Chemical Name:	(R)-3-(3,4-Dimethoxyphenyl)-1-(2-(2-((7-(((S)-1-((2S,4R)-4-hydroxy-2-(((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)amino)-7-oxoheptyl)amino)-2-oxoethoxy)phenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate trifluoroacetate
Purity:	≥98% (HPLC)
Molecular Weight:	1361.58

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Biological Activity for dTAG^V-1

dTAG^V-1 is a degrader targeting mutant FKBP12^F36V fusion proteins. Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a von Hippel-Lindau (VHL)-binding ligand. Induces potent and selective degradation of FKBP12^F36V fusion proteins in vitro and in vivo. Selectively degrades FKBP12^F36V-EWS/FLI fusion proteins and inhibits cell proliferation in FKBP12^F36V-EWS/FLI-expressing Ewing sarcoma cells.

Hydrochloride salt (Cat.No. 7374) available; suitable for in vivo use.

Negative control dTAG^V-1-NEG (Cat. No. 6915) also available.

FKBP12^F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in. Custom knock-in cell lines for the dTAG and aTAG platforms are available from our sister brand R&D Systems. Email TPD@bio-techne.com to enquire.

Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12^F36V are available from Addgene.

Licensing Information

Sold under license from Dana-Farber Cancer Institute

Technical Data for dTAG^V-1

M. Wt	1361.58
Formula	C₆₈H₉₀N₆O₁₄S CF₃CO₂H
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2624313-15-9
InChi Key	KSEWNBIDXKMTNT-LNVAYBNASA-N
Smiles	C[C@H](NC([C@@H]1C[C@@H](O)CN1C([C@@H](NC(CCCCCCNC(COC2=CC=CC=C2[C@H](OC([C@@H]3CCCCN3C([C@@H](CC)C4=CC(OC)=C(OC)C(OC)=C4)=O)=O)CCC5=CC(OC)=C(OC)C=C5)=O)=O)C(C)(C)C)=O)=O)C6=CC=C(C=C6)C7=C(N=CS7)C.OC(C(F)(F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for dTAG^V-1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	136.16	100

Preparing Stock Solutions for dTAG^V-1

The following data is based on the product molecular weight 1361.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.73 mL	3.67 mL	7.34 mL
5 mM	0.15 mL	0.73 mL	1.47 mL
10 mM	0.07 mL	0.37 mL	0.73 mL
50 mM	0.01 mL	0.07 mL	0.15 mL

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References for dTAG^V-1

References are publications that support the biological activity of the product.

Nabet Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat.Commun. 2020 PMID: 32948771

Citations for dTAG^V-1

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dTAG^V-1

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dTAGV-1

Tocris Bioscience a Bio-Techne Brand

Key Product Details

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Biological Activity for dTAGV-1

Licensing Information

Technical Data for dTAGV-1

Solubility for dTAGV-1

Preparing Stock Solutions for dTAGV-1

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for dTAGV-1

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Product Documents for dTAGV-1

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Certificate of Analysis

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Bio-Techne 品牌

dTAG^V-1

Biological Activity for dTAG^V-1

Technical Data for dTAG^V-1

Solubility for dTAG^V-1

Preparing Stock Solutions for dTAG^V-1

References for dTAG^V-1

Citations for dTAG^V-1

Product Documents for dTAG^V-1