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Mouse IgG HRP-conjugated Antibody
Catalog # HAF018 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
HRP
Antibody Source
Polyclonal Donkey IgG
Product Summary for Mouse IgG HRP-conjugated Antibody
Immunogen
Mouse IgG
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Mouse IgG HRP-conjugated Antibody
Detection of Mouse IgG Primary Antibody by Western Blot
Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse IgG HRP-conjugated Antibody
Application
Recommended Usage
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.7 using HAF018 in the following applications:
Published Applications
Read 13 publications using HAF018 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a 0.2 μm filtered solution in PBS with stabilizers.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG
Product Documents for Mouse IgG HRP-conjugated Antibody
Product Specific Notices for Mouse IgG HRP-conjugated Antibody
For research use only
Citations for Mouse IgG HRP-conjugated Antibody (13)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse IgG HRP-conjugated Antibody include:
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Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotMN Akhtar et al. (2022-08-04), Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 Receptor MBio, 2022-013(4):e0330921.
PMID: 35924848 -
Species: N/A
Sample Types: Recombinant Protein
Applications: Western BlotA Tarar et al. (2020-12-01), Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System Methods and protocols, 2020-13(4).
PMID: 33271819 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotA Singhal et al. (2020-05-26), 2-Hydroxypropyl-gamma-cyclodextrin overcomes NPC1 deficiency by enhancing lysosome-ER association and autophagy Sci Rep, 2020-010(1):8663.
PMID: 32457374 -
Species: Mouse
Sample Types: Protein
Applications: ELISA CaptureE Tkaczynski et al. (2018-04-10), 2-O, 3-O desulfated heparin mitigates murine chemotherapy- and radiation-induced thrombocytopenia Blood Adv, 2018-02(7):754-761.
PMID: 29599195 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMF Gutknecht et al. (2017-09-15), Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity Nat Commun, 2017-08(1):552.
PMID: 28916756 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotR Critelli et al. (2017-08-24), Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 2017-08(8):e3017.
PMID: 28837142 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotY Xia et al. (2017-07-19), mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress Oxid Med Cell Longev, 2017-02017(0):6292630.
PMID: 28804534 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Functional AssayP Bokiniec et al. (2017-06-02), Polysialic acid regulates sympathetic outflow by facilitating information transfer within the nucleus of the solitary tract J. Neurosci., 2017-00(0).
PMID: 28576943 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotT Poobalasin et al. (2017-02-24), Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair Dis Model Mech, 2017-00(0).
PMID: 28237967 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot(2016-11-17), Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target Sci Rep, 2016-16(0):37360.
PMID: 27853274 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLynam-Lennon et al. (2014-06-26), Altered mitochondrial function and energy metabolism is associated with a radioresistant phenotype in oesophageal adenocarcinoma. PLoS ONE, 2014-09(6):e100738.
PMID: 24968221 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotKrishnatry AS et al. (2009-10-15), Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin. Nitric Oxide, 2009-122(1):11-7.
PMID: 19836459 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotVink CA et al. (2009-05-05), Sleeping beauty transposition from nonintegrating lentivirus. Mol. Ther., 2009-017(7):1197-204.
PMID: 19417741
There are no citations that match your criteria.
Customer Reviews for Mouse IgG HRP-conjugated Antibody (3)
4.7 out of 5
3 customer
ratings
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Application: Western BlotSample Tested: Tissue Lysates and Colon tissueSpecies: MouseVerified Customer | Posted 11/27/2021
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Application: Western BlotSample Tested: HUVEC human umbilical vein endothelial cells and HeLa cellsSpecies: HumanVerified Customer | Posted 08/05/2020
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Application: Western BlotSample Tested: Muscle tissueSpecies: PigVerified Customer | Posted 08/14/2019
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FAQs for Mouse IgG HRP-conjugated Antibody
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Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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