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Mouse IgG HRP-conjugated Antibody

Catalog # HAF018 | R&D Systems, Inc. a Bio-Techne Brand
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HAF018

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

HRP

Antibody Source

Polyclonal Donkey IgG

Product Summary for Mouse IgG HRP-conjugated Antibody

Immunogen

Mouse IgG

Specificity

Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with human IgG, rabbit IgG and chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Mouse IgG HRP-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Western Blot

Detection of Mouse IgG Primary Antibody by Western Blot

Western blot shows detection of Mouse Anti-Human/Mouse/Rat IGSF4C/SynCAM4 Monoclonal Antibody (Catalog # MAB41642) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IgG HRP-conjugated Antibody

Application
Recommended Usage

Western Blot

Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using HAF018 in the following applications:

Published Applications

Read 13 publications using HAF018 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 μm filtered solution in PBS with stabilizers.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG

Product Documents for Mouse IgG HRP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG HRP-conjugated Antibody

For research use only

Citations for Mouse IgG HRP-conjugated Antibody (13)

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Customer Reviews for Mouse IgG HRP-conjugated Antibody (3)

4.7 out of 5
3 customer ratings
5 stars
67%
4 stars
33%
3 stars
0%
2 stars
0%
1 star
0%

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  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue Lysates and Colon tissue
    Species: Mouse
    Verified Customer | Posted 11/27/2021
    Mouse IgG HRP-conjugated Antibody HAF018
  • Mouse IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HUVEC human umbilical vein endothelial cells and HeLa cells
    Species: Human
    Verified Customer | Posted 08/05/2020
    Mouse IgG HRP-conjugated Antibody HAF018
  • Mouse IgG HRP-conjugated Antibody
    Name: Louise Speed
    Application: Western Blot
    Sample Tested: Muscle tissue
    Species: Pig
    Verified Customer | Posted 08/14/2019
    Mouse IgG HRP-conjugated Antibody HAF018

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FAQs for Mouse IgG HRP-conjugated Antibody

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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