Rabbit anti-Feline IgM Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73920
![Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [NBP1-73920]](https://resources.bio-techne.com/images/products/Rabbit-anti-Feline-IgM-Secondary-Antibody-[Unconjugated]-Flow-Cytometry-NBP1-73920-img0001.jpg "Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [NBP1-73920]")
Key Product Details
Species Reactivity
Feline
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cat IgM mu heavy chain
Specificity
No reaction was observed against Cat IgG.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgM and Cat Serum
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody
Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [NBP1-73920]
Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [NBP1-73920] - Phenotyping of cat blood. Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker. This image was submitted via customer review. Image using the FITC form of this antibody.Applications for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody
Product Specific Notices for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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