Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73839
Key Product Details
Species Reactivity
Feline
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cat IgM mu heavy chain
Specificity
No reaction was observed against Cat IgG.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgM and Cat Serum
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgM and Cat Serum
Applications for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:10000
Application Notes
This product has been assayed against 1.0 ug of Cat IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [HRP]
Product Specific Notices for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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