Recombinant Human VEGF 121 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07103

Novus Biologicals, part of Bio-Techne
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NBP3-07103-500ug
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Key Product Details

Source

Yeast

Accession #

P15692-9

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGF 121 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains corresponding to VEGF 121. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 144-20 kDa in SDS-PAGE under reducing conditions Source: Yeast

Uniprot ID: P15692-9

Amino Acid Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of VEGF 121 as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is between 0.2-0.4 ng/mL.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP3-07103
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, (pH 7.4)
Preservative No Preservative
Concentration Lyoph
Reconstitution Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: VEGF 121

VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. VEGF121 has increased acidity which allowes it to circulate more freely than other VEGF forms. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.

Alternate Names

MVCD1, vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, VEGFMGC70609, VPFvascular endothelial growth factor

Gene Symbol

VEGFA

UniProt

P15692-9 (Human)

Additional VEGF 121 Products

Product Documents for Recombinant Human VEGF 121 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human VEGF 121 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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