Recombinant Rat Integrin alpha V beta 6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9668-AV

R&D Systems, part of Bio-Techne
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9668-AV-050
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Key Product Details

  • R&D Systems CHO-derived Recombinant Rat Integrin alpha V beta 6 Protein (9668-AV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

XP_017456512.1 (Integrin alphaV) & Q6AYF4 (Integrin beta6)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha V beta 6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat Integrin alpha V beta 6 protein
Rat Integrin alphaV
(Phe31-Val987)
Accession # XP_017456512.1		 His-Pro GGGSGGGS Acidic Tail HHHHHH
Rat Integrin beta6
(Gly22-Pro708)
Accession # Q6AYF4		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 (Integrin alphaV) & Gly22 (Integrin beta6)

Predicted Molecular Mass

115 kDa (Integrin alphaV) & 83 kDa (Integrin beta6)

SDS-PAGE

106-153 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Integrin  alphaV beta6 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human LAP (TGF‑ beta1) (Catalog # 246-LP) that produces 50% of the optimal binding response is approximately 0.5‑3 ng/mL.

Scientific Data Images for Recombinant Rat Integrin alpha V beta 6 Protein, CF

Recombinant Rat Integrin alpha V beta 6 Protein Binding Activity

Recombinant Rat Integrin alpha V beta 6 Protein Binding Activity

Immobilized Recombinant Rat Integrin aV beta6 (Catalog # 9668-AV) at 2.0 µg/mL can bind Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) with an ED50 of 0.5-3 ng/mL.

Formulation, Preparation and Storage

9668-AV
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution
Reconstitute at 500 μg/mL in Water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 6

Integrin  alphaV beta6 is one of five alphaV integrins and the sole  beta6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 95 kDa beta6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2-5). The ligand interaction site of alphaV beta6 is in the N-terminal head region formed by an interaction of the beta6 vWFA domain with the alphaV beta-propeller structure (2). The alphaV subunit contains domains termed thigh, calf, and calf-2 with a divalent cation-binding site found at a position equivalent to the "knee". The 958 amino acid (aa) rat alphaV ECD, which is cleaved at aa 886 but remains associated, shares 93% and 98% aa sequence identity with human and mouse  alphaV, while the 687 aa rat beta6 ECD shares 90% and 96% aa sequence identity with human and mouse  beta6, respectively. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta6 C-terminal 11 aa cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP-9 expression (6). Either "inside-out" signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alphaV beta6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF-beta latency-associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes (7). This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta6 ablates tonic inhibition of alveolar macrophages by TGF-beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10-12). High alphaV beta6 expression in carcinomas may contribute to progression through its effects on TGF-beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alphaV beta6 as a receptor, and soluble alphaV beta6 may block virus infectivity in vitro (13, 14).

References

  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Wada, J. et al. (1996) J. Cell Biol. 132:1161.
  5. Arend, L.J. et al. (2000) J. Am. Soc. Nephrol. 11:2297.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.

Alternate Names

CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

XP_017456512.1 (Integrin alphaV) & Q6AYF4 (Integrin beta6)

Additional Integrin alpha V beta 6 Products

Product Documents for Recombinant Rat Integrin alpha V beta 6 Protein, CF

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Product Specific Notices for Recombinant Rat Integrin alpha V beta 6 Protein, CF

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