Recombinant Mouse IL-18/IL-1F4 Protein, CF

Name: Recombinant Mouse IL-18/IL-1F4 Protein, CF
Brand: R&D Systems
SKU: B004-5

R&D Systems, Inc. a Bio-Techne Brand

Catalog # B004-5

Citations (3)

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New Version Available - 9139-IL/CF

A New rm IL-18 is Now Available! Competitively priced; Enhanced stability; Lower endotoxin specification; Made in-house!

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Key Product Details

Source:	E. coli
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-18/IL-1F4 Protein, CF

Product Specifications for Recombinant Mouse IL-18/IL-1F4 Protein, CF

Source	E. coli-derived mouse IL-18/IL-1F4 protein 36-192 aa
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass	18 kDa
Activity	Induction of IFN-gamma by Mouse IL-18 receptor transfected KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant mouse IL-18 was measured using human IFN-gamma ELISA. The activity of lot 040 was as follows:IL-18 final concentration 5ng/mL; IFN-gamma induction 2863 IU/mL and IL-18 final concentration 10 ng/mL; IFN-gamma induction 7312 IU/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

B004-5

Formulation	Lyophilized in PBS containing 1% sucrose.
Reconstitution	Reconstitute in 250 μL of ice-cold distilled water on ice.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8⁺ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4⁺ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4⁺ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).

Item	Value
References	Dinarello, C.A. et al. (2013) Front. Immunol. 4:289. Smith, D.E. (2011) J. Leukoc. Biol. 89:383. Gu, Y. et al. (1997) Science 275:206. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Cheung, H. et al. (2005) J. Immunol. 174:5351. Novick, D. et al. (1999) Immunity 10:127. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769. Lalor, S.J. et al. (2011) J. Immunol. 186:5738. Li, J. et al. (2004) Cell. Immunol. 227:103. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665. Okamura, H. et al. (1995) Nature 378:88.
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Recombinant Mouse IL-18/IL-1F4 Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-18/IL-1F4 Protein, CF include:

Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Munoz M et al. (2015), Interleukin-22 induces interleukin-18 expression from epithelial cells during intestinal infection. Immunity, 42(2):321-31.
PMID: 25680273
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Antonopoulos C et al. (2007), IL-18 is a key proximal mediator of contact hypersensitivity and allergen-induced Langerhans cell migration in murine epidermis. J. Leukoc. Biol., 83(2):361-7.
PMID: 17984289
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay

107335 et al. (2002), STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation. MAD2L2 promotes replication fork protection and recovery in a shieldin-independent and REV3L-dependent manner, 99(19):12281-6.
PMID: 12213961

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