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Recombinant Mouse IFN-alpha 9 Protein, CF

Catalog # 10369-IF | R&D Systems, Inc. a Bio-Techne Brand
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10369-IF-010
10369-IF-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 9 protein
Cys24-Glu190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-24 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is < 100 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 9 Protein, CF

Recombinant Mouse IFN-alpha 9 Protein Bioactivity

Recombinant Mouse IFN-alpha 9 Protein Bioactivity

Recombinant Mouse IFN-alpha 9 (10369-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is
Recombinant Mouse IFN-alpha 9 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 9 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 9 (10369-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.

Formulation, Preparation and Storage

10369-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 9

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). The extracellular domain (ECD) of mature mouse IFNA9 consists of 166 amino acids (aa), with the closest homolog identified in humans as IFNA1. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection. In gB DNA-Vaccinated mice, coimmunisation with IFNA9 has been shown to enhance protective immunity against cytomegalovirus and myocarditis (5).

References

  1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  5. James C.M. et al. (2007) Vaccine. 25(10):1856.
  6. Cull, V.S. et al. (2002) Gene Therapy. 9:1369-78.

Long Name

Interferon alpha 9

Alternate Names

IFA9, IFNA9

Entrez Gene IDs

15972 (Mouse)

Gene Symbol

IFNA9

UniProt

Product Documents for Recombinant Mouse IFN-alpha 9 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-alpha 9 Protein, CF

For research use only

Reconstitution Calculator

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