Recombinant Human SCG3 His-tag Protein, CF

Name: Recombinant Human SCG3 His-tag Protein, CF
Brand: R&D Systems
SKU: 10374-SC

Catalog # 10374-SC | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	CHO
Accession #:	Q8WXD2.3
Applications:	Bioactivity

View Full Technical Details for Recombinant Human SCG3 His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human SCG3 His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human SCG3 protein Phe20-Leu468, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe20
Predicted Molecular Mass	52 kDa
SDS-PAGE	52 kDa
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human SCG3 His-tag (Catalog # 10374-SC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Chromogranin A His-tag (Catalog # 10422-CH) binds with an ED50 of 50-400 ng/mL.

Scientific Data Examples for Recombinant Human SCG3 His-tag Protein, CF

	Recombinant Human SCG3 His-tag Protein Binding Activity When Recombinant Human SCG3 His-tag (10374-SC) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Chromogranin A His-tag (10422-CH) binds with an ED₅₀ of 50-400 ng/mL.
	Recombinant Human SCG3 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human SCG3 His-tag (Catalog # 10374-SC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10374-SC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCG3

Secretogranin III, also known as SCG3, is a member of the chromogranin-secretogranin family of neuroendocrine secretory proteins, collectively referred to as granins (1). Additional members of this family include secretogranin II, IV, and V, chromogranin A and B, and a few additional related proteins (1, 2). SCG3 is synthesized as an N-glycosylated protein and two isoforms can be generated from alternative splicing (2). Mature SCG3 contains three functional regions: an N‑terminal cholesterol-binding domain, a chromogranin A binding domain and a C-terminal carboxypeptidase E binding domain (3). Human SCG3 shares 88% and 87% amino acid sequence identity with mouse and rat SCG3, respectively. Granins are highly acidic proteins that are a major component of secretory granules of endocrine, neuroendocrine, and neuronal cells (4-6). Calcium binding at low pH and intermolecular aggregation plays a role in granins' sorting of secretory proteins at the trans-Golgi network (3-6). Within the trans-Golgi network, SCG3 binds to chromogranin A (CgA), membrane-associated carboxypeptidase E (CPE), and cholesterol‑rich membrane domain (3-5). SCG3 has been implicated in diabetic retinopathy (DR) and retinopathy of prematurity (ROP) as a disease-associated endothelial ligand, thus suggesting SCG3 is a promising target for novel antiangiogenic therapy of DR and ROP (7, 8).

Item	Value
References	Peinado, J. R. et al. (2006) Endocrinology. 147:1408. Bartolomucci, A. et al. (2011) Endocr. Rev. 32:755. Hosaka M. et al. (2010) Endocr. J. 57:275. Sun, M. et al. (2013) Traffic. 14:205. Hosaka, M. et al. (2004) J. Biol. Chem. 279:3627. Hosaka, M. et al. (2005) J. Cell Sci. 118:4785. LeBlanc, M. E. et al. (2017) J. Exp. Med. 214:1029. Tang, F. et al. (2017) Biochem. Biophys. Res. Commun. 495:781.
Long Name	Secretogranin III
Entrez Gene IDs	29106 (Human); 20255 (Mouse)
Alternate Names	SCG3, SGIII, Secretogranin III, Secretogranin-3, secretogranin IIIFLJ90833

Citations for Recombinant Human SCG3 His-tag Protein, CF

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Recombinant Human SCG3 His-tag Protein, CF

Catalog # 10374-SC | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human SCG3 His-tag Protein, CF

Scientific Data Examples for Recombinant Human SCG3 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: SCG3

Citations for Recombinant Human SCG3 His-tag Protein, CF

Product Documents for Recombinant Human SCG3 His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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