Recombinant Human PVRIG Fc Chimera Protein, CF

Name: Recombinant Human PVRIG Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9365-PV
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9365-PV

Citations (1)

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Key Product Details

Source:	HEK293
Accession #:	Q6DKI7
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PVRIG Fc Chimera Protein, CF

Product Specifications for Recombinant Human PVRIG Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human PVRIG protein

Human PVRIG (Thr41 & Glu43-Leu172) Accession # Q6DKI7	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr41 & Glu43

Predicted Molecular Mass

40 kDa

SDS-PAGE

51-57 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human PVRIG Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human
Nectin-2/CD112 (Catalog # 2229-N2) that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.

Scientific Data Examples for Recombinant Human PVRIG Fc Chimera Protein, CF

	Recombinant Human PVRIG Fc Chimera Protein Bioactivity When Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds with an ED₅₀ of 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9365-PV

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and PEG with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in sterile water. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

Item	Value
References	Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.
Long Name	Poliovirus Receptor Related Immunoglobulin Domain Containing
Entrez Gene IDs	79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)
Alternate Names	C7orf15, C7orf15MGC138295, CD112R, MGC104322, MGC138297, MGC2463, PVRIG, Poliovirus receptor-related immunoglobulin domain-containing protein, poliovirus receptor related immunoglobulin domain containing, transmembrane protein PVRIG

Citations for Recombinant Human PVRIG Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PVRIG Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Joung et al. (2022), CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 13(1):1606.
PMID: 35338135

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Product Documents for Recombinant Human PVRIG Fc Chimera Protein, CF

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