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Recombinant Human PANK2 His-tag Protein, CF

Catalog # 10664-PK | R&D Systems, Inc. a Bio-Techne Brand
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10664-PK-020

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human PANK2 protein
Lys206-Pro570, with C-terminal 6-His

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Lys206

Predicted Molecular Mass

41.6 kD

SDS-PAGE

39 kD

Activity

Measured by its ability to transfer phosphate from ATP to pantothenic acid.
The specific activity is >80 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human PANK2 His-tag Protein, CF

Recombinant Human PANK2 His-tag Protein Enzyme Activity Diagram

PANK2 catalyzes the first committed step in the pathway leading to the biosynthesis of coenzyme A. PANK2 is also a key regulatory enzyme in this pathway.

Recombinant Human PANK2 His-tag SDS-PAGE.

1 μg/lane of Recombinant Human PANK2 His-tag (Catalog # 10664-PK) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 39 kDa.

Formulation, Preparation and Storage

10664-PK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PANK2

The pantothenate kinase (PANK) gene family contains four members and encode five functional isoforms. The PANK enzymes are key to the synthesis of coenzyme A (CoA) (1,2). CoA is used by around 4% of intracellular enzymes as a co-substrate. PANK2, the only member of the PANK family that resides in mitochondria, is feedback regulated by CoA and may be the master regulator of CoA synthesis (3, 4). Mutations in PANK2 results in Hallervorden-Spatz syndrome, an autosomal recessive neurodegenerative disorder caused by excessive brain iron accumulation due to the defects in CoA synthesis (1,5). The activity of this enzyme has been measured with a phosphatase-coupled method (6).

References

  1. Zhou, B. et al. (2001) Nat Genet. 28:345.
  2. Polsterm B.J. et al. (2010) Gene  465:53.
  3. Rock, C.O. et al. (2000) J Biol Chem. 275:1377.
  4. Srinivasan, B. et al. (2015) Nat Chem Biol. 11:784.
  5. Orellana, D.I. et al. (2016) EMBO Mol Med. 8:1197.
  6. Wu, Z.L. et al. (2011) PLoS ONE 6:e23172.

Long Name

Pantothenate Kinase 2

Alternate Names

FLJ11729, HPANK2, NBIA1, PKAN

Entrez Gene IDs

80025 (Human); 74450 (Mouse)

Gene Symbol

PANK2

UniProt

Product Documents for Recombinant Human PANK2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PANK2 His-tag Protein, CF

For research use only

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