Recombinant Human Mrc2 His-tag Protein, CF

Catalog # 10483-EN | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

AAF14192.1

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Mrc2 protein
Gly31-Ala1414, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly31

Predicted Molecular Mass

157 kDa

SDS-PAGE

157-203 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Mrc2 His-tag (Catalog # 10483-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha1  (Catalog # 6220-CL) binds with an ED50 of 0.03-0.36 μg/mL.

Scientific Data Images for Recombinant Human Mrc2 His-tag Protein, CF

Recombinant Human Mrc2 His-tag Protein Binding Activity

Recombinant Human Mrc2 His-tag Protein Binding Activity

When Recombinant Human Mrc2 His-tag (10483-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha1 (6220-CL) binds with an ED50 of 0.03-0.36 μg/mL.
Recombinant Human Mrc2 His-tag Protein SDS-PAGE

Recombinant Human Mrc2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Mrc2 His-tag (10483-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 155-205 kDa.

Formulation, Preparation and Storage

10483-EN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2),  also know as MMR2, Endocytic Receptor 180 and uPARAP,  is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2.  Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

  1. Yuan, C. et al. (2016) Biochem. J. 473:2359.
  2. Durrel, T. et al. (2018) Nat. Commun. 9:5178.
  3. Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
  4. Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Alternate Names

CD280, CLEC13E, Endo180, uPARAP

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Gene Symbol

MRC2

UniProt

AAF14192.1

Product Documents for Recombinant Human Mrc2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Mrc2 His-tag Protein, CF

For research use only

Reconstitution Calculator

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