Recombinant Human Matrilin-2 Protein

Name: Recombinant Human Matrilin-2 Protein
Brand: R&D Systems
SKU: 3044-MN
Price: 476 USD

Catalog # 3044-MN | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	AAH10444
Applications:	Binding Activity

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Product Specifications for Recombinant Human Matrilin-2 Protein

Source	Mouse myeloma cell line, NS0-derived human Matrilin-2 protein Arg24-Arg937, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Arg24
Predicted Molecular Mass	103 kDa
SDS-PAGE	120 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized rat tail Collagen I at 10 µg/mL (100 µL/well) can bind rhMatrilin-2 with a linear range of 16-1,000 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

3044-MN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3044-MN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Matrilin-2

Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix proteins (1 - 4). The human Matrilin-2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C-terminal coiled-coil domain (5, 6). Alternate splicing generates Isoform 2 (with an 18 aa deletion near the C-terminus), Isoform 3 (with a deletion of the fourth EGF-like repeat), and Isoform 4 (with a deletion of the first VWA and first EGF-like repeat). Human Matrilin-2 shares 87% and 84% aa sequence identity with mouse and canine Matrilin-2, respectively, and 27%, 22%, and 33% aa sequence identity with human Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled-coil domain (4, 7, 8, 9). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 7, 8) but has not been detected in heterotrimers containing Matrilin-3 (8). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrillin-2, and Laminin-1/Nidogen-1 complexes (7). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (10).

Item	Value
References	Wagener, R. et al. (2005) FEBS Lett. 579:3323. Deak, F. et al. (1999) Matrix Biol. 18:55. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369. Piecha, D. et al. (1999) J. Biol. Chem. 274:13353. Muratoglu, S. et al. (2000) Cytogenet. Cell Genet. 90:323. Deak, F. et al. (1997) J. Biol. Chem. 272:9268. Piecha, D. et al. (2002) Biochem. J. 367:715. Frank, S. et al. (2002) J. Biol. Chem. 277:19071. Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205. Mates, L. et al. (2004) Matrix Biol. 23:195.
Entrez Gene IDs	4147 (Human)
Alternate Names	MATN2, Matrilin-2, Matrilin2, matrilin 2

Citations for Recombinant Human Matrilin-2 Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Matrilin-2 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E The et al. (2022-08-29), Interleukin 38 alleviates aortic valve calcification by inhibition of NLRP3 Proceedings of the National Academy of Sciences of the United States of America, 2022-0119(36):e2202577119.
PMID: 36037361
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

E The et al. (2020-02-07), Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells Cells, 2020-09(2).
PMID: 32046115
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Li et al. (2017-05-25), ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells Arterioscler. Thromb. Vasc. Biol., 2017-00(0).
PMID: 28546218

Recombinant Human Matrilin-2 Protein

Catalog # 3044-MN | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Matrilin-2 Protein

Formulation, Preparation and Storage

Carrier Free

3044-MN

Carrier

3044-MN/CF

Carrier Free

Reconstitution Calculator

Background: Matrilin-2

Citations for Recombinant Human Matrilin-2 Protein (3)

Product Documents for Recombinant Human Matrilin-2 Protein

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